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Galectin 3 antibody [024]

Anti-Galectin 3 antibody [024] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02037
Anti-Galectin 3 antibody [024] used in Flow cytometry (FACS). GTX02037
Anti-Galectin 3 antibody [024] used in Western Blot (WB). GTX02037
Anti-Galectin 3 antibody [024] used in Immunoprecipitation (IP). GTX02037

Cat No. GTX02037

Host

Rabbit

Clonality

Monoclonal

Clone Name

024

Isotype

IgG

Application

WB, ICC/IF, FACS, IP

Reactivity

Human, Mouse
Package
100 μl ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:2000
ICC/IF 1:20-1:100
FACS 1:25-1:100
IP 0.5-2 μl/mg of lysate
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.2 μm filtered PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant Human Galectin-3 / LGALS3 protein

Purification

Purified by Protein A

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

galectin 3 , CBP35 , GAL3 , GALBP , GALIG , L31 , LGALS2 , MAC2

Cellular Localization

Cytoplasm , Nucleus , Secreted

Background

This gene encodes a member of the galectin family of carbohydrate binding proteins. Members of this protein family have an affinity for beta-galactosides. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. This protein can self-associate through the N-terminal domain allowing it to bind to multivalent saccharide ligands. This protein localizes to the extracellular matrix, the cytoplasm and the nucleus. This protein plays a role in numerous cellular functions including apoptosis, innate immunity, cell adhesion and T-cell regulation. The protein exhibits antimicrobial activity against bacteria and fungi. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Oct 2014]

Database

Research Area

DATA IMAGES

Anti-Galectin 3 antibody [024] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02037

GTX02037 ICC/IF Image

ICC/IF analysis of PFA-fixed HeLa cells using GTX02037 Galectin 3 antibody [024].
Green : Primary antibody
Dilution : 1:60
Permeabilzation : 1% Triton X-100

Anti-Galectin 3 antibody [024] used in Flow cytometry (FACS). GTX02037

GTX02037 FACS Image

FACS analysis of HeLa cells using GTX02037 Galectin 3 antibody [024].
The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

Anti-Galectin 3 antibody [024] used in Western Blot (WB). GTX02037

GTX02037 WB Image

WB analysis of various samples using GTX02037 Galectin 3 antibody [024].
Lane A : HeLa whole cell lysate
Lane B : SW480 whole cell lysate
Lane C : A431 whole cell lysate
Dilution : 1:500
Loading : 30 μg

Anti-Galectin 3 antibody [024] used in Immunoprecipitation (IP). GTX02037

GTX02037 IP Image

IP analysis of mouse pancreas whole cell lysate using GTX02037 Galectin 3 antibody [024].
IP antibody : 4 μl per 0.5 mg total whole cell lysate
Dilution : 1:100
Immunomagnetic beads Protein A/G : 60 μg

REFERENCE

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REVIEW

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