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Galectin 3 antibody [A3A12]

Anti-Galectin 3 antibody [A3A12] used in IHC (Paraffin sections) (IHC-P). GTX22785
Anti-Galectin 3 antibody [A3A12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22785
Anti-Galectin 3 antibody [A3A12] used in IHC (Frozen sections) (IHC-Fr). GTX22785
Anti-Galectin 3 antibody [A3A12] used in IHC (Paraffin sections) (IHC-P). GTX22785
Anti-Galectin 3 antibody [A3A12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22785
Anti-Galectin 3 antibody [A3A12] used in IHC (Paraffin sections) (IHC-P). GTX22785
Anti-Galectin 3 antibody [A3A12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22785
Anti-Galectin 3 antibody [A3A12] used in Western Blot (WB). GTX22785

Cat No. GTX22785

Host

Mouse

Clonality

Monoclonal

Clone Name

A3A12

Isotype

IgG1

Application

WB, ICC/IF, IHC-P, IHC-Fr, ELISA

Reactivity

Human, Mouse, Rat
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 μg/ml
ICC/IF 1:20-1:200
IHC-P 1:20-1:200
IHC-Fr 6 μg/ml
ELISA Assay dependent
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

Product Note

The epitope for this antibody has been mapped to the first 58 amino acids of galectin-3.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length recombinant human galectin-3.

Purification

Purified IgG

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

galectin 3 , CBP35 , GAL3 , GALBP , GALIG , L31 , LGALS2 , MAC2

Cellular Localization

Cytoplasm,Nucleus,Secreted

Background

This gene encodes a member of the galectin family of carbohydrate binding proteins. Members of this protein family have an affinity for beta-galactosides. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. This protein can self-associate through the N-terminal domain allowing it to bind to multivalent saccharide ligands. This protein localizes to the extracellular matrix, the cytoplasm and the nucleus. This protein plays a role in numerous cellular functions including apoptosis, innate immunity, cell adhesion and T-cell regulation. The protein exhibits antimicrobial activity against bacteria and fungi. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Oct 2014]

Database

Research Area

DATA IMAGES

Anti-Galectin 3 antibody [A3A12] used in IHC (Paraffin sections) (IHC-P). GTX22785

GTX22785 IHC-P Image

IHC-P analysis of human ovarian carcinoma tissue using GTX22785 Galectin 3 antibody [A3A12].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0) microwaved for 8-15 min
Dilution : 1:100

Anti-Galectin 3 antibody [A3A12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22785

GTX22785 ICC/IF Image

ICC/IF analysis of NIH-3T3 cells using GTX22785 Galectin 3 antibody [A3A12]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:100 incubated overnight at 4 ºC

Anti-Galectin 3 antibody [A3A12] used in IHC (Frozen sections) (IHC-Fr). GTX22785

GTX22785 IHC-Fr Image

IHC-Fr analysis of rat olfactory bulb tissue using GTX22785 Galectin 3 antibody [A3A12].

Anti-Galectin 3 antibody [A3A12] used in IHC (Paraffin sections) (IHC-P). GTX22785

GTX22785 IHC-P Image

IHC-P analysis of mouse colon tissue using GTX22785 Galectin 3 antibody [A3A12].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0) microwaved for 8-15 min
Dilution : 1:20

Anti-Galectin 3 antibody [A3A12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22785

GTX22785 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX22785 Galectin 3 antibody [A3A12]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:100 incubated overnight at 4 ºC

Anti-Galectin 3 antibody [A3A12] used in IHC (Paraffin sections) (IHC-P). GTX22785

GTX22785 IHC-P Image

IHC-P analysis of human colon tissue using GTX22785 Galectin 3 antibody [A3A12].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0) microwaved for 8-15 min
Dilution : 1:100

Anti-Galectin 3 antibody [A3A12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22785

GTX22785 ICC/IF Image

ICC/IF analysis of MCF-7 cells using GTX22785 Galectin 3 antibody [A3A12]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:100 incubated overnight at 4 ºC

Anti-Galectin 3 antibody [A3A12] used in Western Blot (WB). GTX22785

GTX22785 WB Image

WB analysis of 25 ug of MCF-7 (Lane 1), Hela (Lane 2), and NIH-3T3 cell lysates (Lane 3) using GTX22785 Galectin 3 antibody [A3A12].
Dilution : 1:1000

REFERENCE

There are currently no references for Galectin 3 antibody [A3A12] (GTX22785). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for Galectin 3 antibody [A3A12] (GTX22785). Be the first to share your experience with this product.
SDS
PBS.pdf
Sodium Azide.pdf