APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1 μg/ml |
| 1:20-1:200 |
| 1:20-1:200 |
| 6 μg/ml |
| Assay dependent |
Not tested in other applications.
Calculated MW
Product Note
The epitope for this antibody has been mapped to the first 58 amino acids of galectin-3.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Full length recombinant human galectin-3.
Purification
Purified IgG
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
galectin 3 , CBP35 , GAL3 , GALBP , GALIG , L31 , LGALS2 , MAC2
Cellular Localization
Cytoplasm,Nucleus,Secreted
Background
This gene encodes a member of the galectin family of carbohydrate binding proteins. Members of this protein family have an affinity for beta-galactosides. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. This protein can self-associate through the N-terminal domain allowing it to bind to multivalent saccharide ligands. This protein localizes to the extracellular matrix, the cytoplasm and the nucleus. This protein plays a role in numerous cellular functions including apoptosis, innate immunity, cell adhesion and T-cell regulation. The protein exhibits antimicrobial activity against bacteria and fungi. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Oct 2014]
Database
Research Area
DATA IMAGES
![Anti-Galectin 3 antibody [A3A12] used in IHC (Paraffin sections) (IHC-P). GTX22785](/templates/default/js/lightbox/images/loading.gif "Anti-Galectin 3 antibody [A3A12] used in IHC (Paraffin sections) (IHC-P). GTX22785")
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GTX22785 IHC-P Image
IHC-P analysis of human ovarian carcinoma tissue using GTX22785 Galectin 3 antibody [A3A12].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0) microwaved for 8-15 min
Dilution : 1:100
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GTX22785 IHC-P Image
IHC-P analysis of human colon tissue using GTX22785 Galectin 3 antibody [A3A12].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0) microwaved for 8-15 min
Dilution : 1:100
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GTX22785 ICC/IF Image
ICC/IF analysis of MCF-7 cells using GTX22785 Galectin 3 antibody [A3A12]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:100 incubated overnight at 4 ºC
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GTX22785 IHC-P Image
IHC-P analysis of mouse colon tissue using GTX22785 Galectin 3 antibody [A3A12].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0) microwaved for 8-15 min
Dilution : 1:20
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GTX22785 WB Image
WB analysis of 25 ug of MCF-7 (Lane 1), Hela (Lane 2), and NIH-3T3 cell lysates (Lane 3) using GTX22785 Galectin 3 antibody [A3A12].
Dilution : 1:1000
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GTX22785 IHC-Fr Image
IHC-Fr analysis of rat olfactory bulb tissue using GTX22785 Galectin 3 antibody [A3A12].
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GTX22785 ICC/IF Image
ICC/IF analysis of NIH-3T3 cells using GTX22785 Galectin 3 antibody [A3A12]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:100 incubated overnight at 4 ºC
|
|
|
GTX22785 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX22785 Galectin 3 antibody [A3A12]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:100 incubated overnight at 4 ºC
|
REFERENCE
There are currently no references for Galectin 3 antibody [A3A12] (GTX22785). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Galectin 3 antibody [A3A12] (GTX22785). Be the first to share your experience with this product.
![Anti-Galectin 3 antibody [A3A12] used in IHC (Paraffin sections) (IHC-P). GTX22785](/upload/website/prouct_img/small/GTX22785/GTX22785_1108_IHC-P_w_23060620_715.webp "Anti-Galectin 3 antibody [A3A12] used in IHC (Paraffin sections) (IHC-P). GTX22785")
![Anti-Galectin 3 antibody [A3A12] used in IHC (Paraffin sections) (IHC-P). GTX22785](/upload/website/prouct_img/small/GTX22785/GTX22785_1107_IHC-P_w_23060620_345.webp "Anti-Galectin 3 antibody [A3A12] used in IHC (Paraffin sections) (IHC-P). GTX22785")
![Anti-Galectin 3 antibody [A3A12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22785](/upload/website/prouct_img/small/GTX22785/GTX22785_436_ICC-IF_w_23060620_833.webp "Anti-Galectin 3 antibody [A3A12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22785")
![Anti-Galectin 3 antibody [A3A12] used in IHC (Paraffin sections) (IHC-P). GTX22785](/upload/website/prouct_img/small/GTX22785/GTX22785_1109_IHC-P_w_23060620_285.webp "Anti-Galectin 3 antibody [A3A12] used in IHC (Paraffin sections) (IHC-P). GTX22785")
![Anti-Galectin 3 antibody [A3A12] used in Western Blot (WB). GTX22785](/upload/website/prouct_img/small/GTX22785/GTX22785_1591_WB_w_23060620_665.webp "Anti-Galectin 3 antibody [A3A12] used in Western Blot (WB). GTX22785")
![Anti-Galectin 3 antibody [A3A12] used in IHC (Frozen sections) (IHC-Fr). GTX22785](/upload/website/prouct_img/small/GTX22785/GTX22785_960_IHC-Fr_w_23060620_148.webp "Anti-Galectin 3 antibody [A3A12] used in IHC (Frozen sections) (IHC-Fr). GTX22785")
![Anti-Galectin 3 antibody [A3A12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22785](/upload/website/prouct_img/small/GTX22785/GTX22785_437_ICC-IF_w_23060620_225.webp "Anti-Galectin 3 antibody [A3A12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22785")
![Anti-Galectin 3 antibody [A3A12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22785](/upload/website/prouct_img/small/GTX22785/GTX22785_438_ICC-IF_w_23060620_588.webp "Anti-Galectin 3 antibody [A3A12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22785")
![Anti-Galectin 3 antibody [A3A12] used in IHC (Paraffin sections) (IHC-P). GTX22785](/upload/website/prouct_img/normal/GTX22785/GTX22785_1108_IHC-P_w_23060620_715.webp "Anti-Galectin 3 antibody [A3A12] used in IHC (Paraffin sections) (IHC-P). GTX22785")
![Anti-Galectin 3 antibody [A3A12] used in IHC (Paraffin sections) (IHC-P). GTX22785](/upload/website/prouct_img/normal/GTX22785/GTX22785_1107_IHC-P_w_23060620_345.webp "Anti-Galectin 3 antibody [A3A12] used in IHC (Paraffin sections) (IHC-P). GTX22785")
![Anti-Galectin 3 antibody [A3A12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22785](/upload/website/prouct_img/normal/GTX22785/GTX22785_436_ICC-IF_w_23060620_833.webp "Anti-Galectin 3 antibody [A3A12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22785")
![Anti-Galectin 3 antibody [A3A12] used in IHC (Paraffin sections) (IHC-P). GTX22785](/upload/website/prouct_img/normal/GTX22785/GTX22785_1109_IHC-P_w_23060620_285.webp "Anti-Galectin 3 antibody [A3A12] used in IHC (Paraffin sections) (IHC-P). GTX22785")
![Anti-Galectin 3 antibody [A3A12] used in Western Blot (WB). GTX22785](/upload/website/prouct_img/normal/GTX22785/GTX22785_1591_WB_w_23060620_665.webp "Anti-Galectin 3 antibody [A3A12] used in Western Blot (WB). GTX22785")
![Anti-Galectin 3 antibody [A3A12] used in IHC (Frozen sections) (IHC-Fr). GTX22785](/upload/website/prouct_img/normal/GTX22785/GTX22785_960_IHC-Fr_w_23060620_148.webp "Anti-Galectin 3 antibody [A3A12] used in IHC (Frozen sections) (IHC-Fr). GTX22785")
![Anti-Galectin 3 antibody [A3A12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22785](/upload/website/prouct_img/normal/GTX22785/GTX22785_437_ICC-IF_w_23060620_225.webp "Anti-Galectin 3 antibody [A3A12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22785")
![Anti-Galectin 3 antibody [A3A12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22785](/upload/website/prouct_img/normal/GTX22785/GTX22785_438_ICC-IF_w_23060620_588.webp "Anti-Galectin 3 antibody [A3A12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22785")