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Galectin 3 antibody [GT1188]

KO/KD
Anti-Galectin 3 antibody [GT1188] used in Western Blot (WB). GTX635156
Anti-Galectin 3 antibody [GT1188] used in Western Blot (WB). GTX635156
Anti-Galectin 3 antibody [GT1188] used in ELISA (ELISA). GTX635156
Anti-Galectin 3 antibody [GT1188] used in Western Blot (WB). GTX635156
Anti-Galectin 3 antibody [GT1188] used in IHC (Paraffin sections) (IHC-P). GTX635156

Cat. No. GTX635156

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT1188

Isotype

 IgG1

Application

 WB, IHC-P, ELISA, Sandwich ELISA

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Galectin 3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
ELISA Assay dependent
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture : GTX635156, Detection: GTX125897
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

Positive Control

HCT-116 , A431 , HeLa

Predict Reactivity

Mouse, Rat, Rabbit, Bovine, Dog, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Galectin 3. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888504

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

galectin 3 , CBP35 , GAL3 , GALBP , GALIG , L31 , LGALS2 , MAC2

Cellular Localization

Cytoplasm,Nucleus,Secreted

Background

This gene encodes a member of the galectin family of carbohydrate binding proteins. Members of this protein family have an affinity for beta-galactosides. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. This protein can self-associate through the N-terminal domain allowing it to bind to multivalent saccharide ligands. This protein localizes to the extracellular matrix, the cytoplasm and the nucleus. This protein plays a role in numerous cellular functions including apoptosis, innate immunity, cell adhesion and T-cell regulation. The protein exhibits antimicrobial activity against bacteria and fungi. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Oct 2014]

Database

Research Area

DATA IMAGES

Anti-Galectin 3 antibody [GT1188] used in Western Blot (WB). GTX635156

GTX635156 WB Image

KO/KD

Wild-type (WT) and Galectin 3 knockout (KO) HeLa cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Galectin 3 antibody [GT1188] (GTX635156) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Galectin 3 antibody [GT1188] used in Western Blot (WB). GTX635156

GTX635156 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Galectin 3 antibody [GT1188] (GTX635156) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Galectin 3 antibody [GT1188] used in ELISA (ELISA). GTX635156

GTX635156 ELISA Image

Sandwich ELISA detection of recombinant full-length Galectin 3 protein using GTX635156 as capture antibody at concentration of 5 μg/mL and GTX125897 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-Galectin 3 antibody [GT1188] used in Western Blot (WB). GTX635156

GTX635156 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Galectin 3 antibody [GT1188] (GTX635156) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Galectin 3 antibody [GT1188] used in IHC (Paraffin sections) (IHC-P). GTX635156

GTX635156 IHC-P Image

Galectin 3 antibody [GT1188] detects Galectin 3 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human colon.
Galectin 3 stained by Galectin 3 antibody [GT1188] (GTX635156) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169