Galectin 3 antibody [GT12712]

Wild-type (WT) and Galectin 3 knockout (KO) HeLa cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Galectin 3 antibody [GT12712] (GTX635155) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Galectin 3 antibody [GT12712] detects Galectin 3 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human colon.
Galectin 3 stained by Galectin 3 antibody [GT12712] (GTX635155) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Galectin 3 antibody [GT12712] (GTX635155) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Galectin 3 antibody [GT12712] detects Galectin 3 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Galectin 3 stained by Galectin 3 antibody [GT12712] (GTX635155) diluted at 1:200.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

Sandwich ELISA detection of recombinant full-length Galectin 3 protein using GTX635155 as capture antibody at concentration of 5 μg/mL and GTX125897 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.