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Galectin 3 antibody [GT1473]

Anti-Galectin 3 antibody [GT1473] used in IHC (Paraffin sections) (IHC-P). GTX635151
Anti-Galectin 3 antibody [GT1473] used in Western Blot (WB). GTX635151
Anti-Galectin 3 antibody [GT1473] used in ELISA (ELISA). GTX635151

Cat No. GTX635151

Host

Mouse

Clonality

Monoclonal

Clone Name

GT1473

Isotype

IgG1

Application

WB, IHC-P, ELISA, Sandwich ELISA

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
ELISA Assay dependent
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture : GTX635151, Detection: GTX113486

Not tested in other applications.

Calculated MW

26 kDa. ( Note )

Positive Control

AsPC-1

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.35 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Galectin 3. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.
PBS

Conjugation

Unconjugated

RRID

AB_2888501

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

galectin 3 , CBP35 , GAL3 , GALBP , GALIG , L31 , LGALS2 , MAC2

Cellular Localization

Cytoplasm,Nucleus,Secreted

Background

This gene encodes a member of the galectin family of carbohydrate binding proteins. Members of this protein family have an affinity for beta-galactosides. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. This protein can self-associate through the N-terminal domain allowing it to bind to multivalent saccharide ligands. This protein localizes to the extracellular matrix, the cytoplasm and the nucleus. This protein plays a role in numerous cellular functions including apoptosis, innate immunity, cell adhesion and T-cell regulation. The protein exhibits antimicrobial activity against bacteria and fungi. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Oct 2014]

Database

Research Area

DATA IMAGES

Anti-Galectin 3 antibody [GT1473] used in IHC (Paraffin sections) (IHC-P). GTX635151

GTX635151 IHC-P Image

Galectin 3 antibody [GT1473] detects Galectin 3 protein by immunohistochemical analysis.
Sample: Paraffin-embedded human colon.
Galectin 3 stained by Galectin 3 antibody [GT1473] (GTX635151) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Galectin 3 antibody [GT1473] used in Western Blot (WB). GTX635151

GTX635151 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Galectin 3 antibody [GT1473] (GTX635151) diluted at 1:1000. The HRP-conjugated anti-mouset IgG antibody (GTX213111-01) was used to detect the primary antibody.
The observed expression level is based on the publication: PMID: 29274868

Anti-Galectin 3 antibody [GT1473] used in ELISA (ELISA). GTX635151

GTX635151 ELISA Image

Sandwich ELISA detection of recombinant full-length Galectin 3 protein using GTX635151 as capture antibody at concentration of 5 μg/mL and GTX113486 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf