APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
1:100-1:1000 |
Assay dependent |
Assay dependent |
Note :
Sandwich ELISA
Capture : GTX635151, Detection: GTX113486
|
Not tested in other applications.
Calculated MW
Predict Reactivity
Mouse, Rat, Rabbit, Bovine, Dog, Pig, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.35 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human Galectin 3. The exact sequence is proprietary.
Purification
Affinity purified by Protein G.
PBS
Conjugation
Unconjugated
RRID
AB_2888501
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
galectin 3 , CBP35 , GAL3 , GALBP , GALIG , L31 , LGALS2 , MAC2
Cellular Localization
Cytoplasm,Nucleus,Secreted
Background
This gene encodes a member of the galectin family of carbohydrate binding proteins. Members of this protein family have an affinity for beta-galactosides. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. This protein can self-associate through the N-terminal domain allowing it to bind to multivalent saccharide ligands. This protein localizes to the extracellular matrix, the cytoplasm and the nucleus. This protein plays a role in numerous cellular functions including apoptosis, innate immunity, cell adhesion and T-cell regulation. The protein exhibits antimicrobial activity against bacteria and fungi. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Oct 2014]
Database
Research Area
DATA IMAGES
|
GTX635151 WB Image
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Galectin 3 antibody [GT1473] (GTX635151) diluted at 1:1000. The HRP-conjugated anti-mouset IgG antibody (GTX213111-01) was used to detect the primary antibody.
The observed expression level is based on the publication: PMID: 29274868
|
|
GTX635151 IHC-P Image
Galectin 3 antibody [GT1473] detects Galectin 3 protein by immunohistochemical analysis.Sample: Paraffin-embedded human colon.Galectin 3 stained by Galectin 3 antibody [GT1473] (GTX635151) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
|
GTX635151 ELISA Image
Sandwich ELISA detection of recombinant full-length Galectin 3 protein using GTX635151 as capture antibody at concentration of 5 μg/mL and GTX113486 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.
|
REFERENCE
There are currently no references for Galectin 3 antibody [GT1473] (GTX635151). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Galectin 3 antibody [GT1473] (GTX635151). Be the first to share your experience with this product.