Galectin 3 antibody [GT759]

Cat. No. GTX635152

Host	Mouse
Clonality	Monoclonal
Clone Name	GT759
Isotype	IgG1
Application	WB, ELISA, Sandwich ELISA
Reactivity	Human
Package	100 μl ($399), 25 μl ($169)

See all Galectin 3 products

Datasheet
Document

View product citations for antibody GTX635152 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ELISA	Assay dependent
Sandwich ELISA	Assay dependent
Note : Sandwich ELISA Capture : GTX635152, Detection: GTX113486

Not tested in other applications.

Calculated MW

26 kDa. ( Note )

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.66 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Galectin 3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.
PBS

Conjugation

Unconjugated

RRID

AB_2888502

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

galectin 3 , CBP35 , GAL3 , GALBP , GALIG , L31 , LGALS2 , MAC2

Cellular Localization

Cytoplasm,Nucleus,Secreted

Background

This gene encodes a member of the galectin family of carbohydrate binding proteins. Members of this protein family have an affinity for beta-galactosides. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. This protein can self-associate through the N-terminal domain allowing it to bind to multivalent saccharide ligands. This protein localizes to the extracellular matrix, the cytoplasm and the nucleus. This protein plays a role in numerous cellular functions including apoptosis, innate immunity, cell adhesion and T-cell regulation. The protein exhibits antimicrobial activity against bacteria and fungi. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Oct 2014]

Database

Gene ID: 3958 LGALS3
UniProt: P17931 LGALS3

Research Area

DATA IMAGES

GTX635152 ELISA Image

Sandwich ELISA detection of recombinant full-length Galectin 3 protein using GTX635152 as capture antibody at concentration of 5 μg/mL and GTX113486 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

GTX635152 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Galectin 3 antibody [GT759] (GTX635152) diluted at 1:1000. The HRP-conjugated anti-mouset IgG antibody (GTX213111-01) was used to detect the primary antibody.
The observed expression level is based on the publication: PMID: 29274868

REFERENCE

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REVIEW

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SDS
PBS.pdf

Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty