APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| Assay dependent |
Not tested in other applications.
Calculated MW
Predict Reactivity
Rat, Rabbit, Bovine, Dog, Pig, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fragment of human Galectin 3
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
galectin 3 , CBP35 , GAL3 , GALBP , GALIG , L31 , LGALS2 , MAC2
Cellular Localization
Cytoplasm,Nucleus,Secreted
Background
This gene encodes a member of the galectin family of carbohydrate binding proteins. Members of this protein family have an affinity for beta-galactosides. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. This protein can self-associate through the N-terminal domain allowing it to bind to multivalent saccharide ligands. This protein localizes to the extracellular matrix, the cytoplasm and the nucleus. This protein plays a role in numerous cellular functions including apoptosis, innate immunity, cell adhesion and T-cell regulation. The protein exhibits antimicrobial activity against bacteria and fungi. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Oct 2014]
Database
Research Area
DATA IMAGES
![Anti-Galectin 3 antibody [HL1878] used in Western Blot (WB). GTX637627](/templates/default/js/lightbox/images/loading.gif "Anti-Galectin 3 antibody [HL1878] used in Western Blot (WB). GTX637627")
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GTX637627 WB Image
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Galectin 3 antibody [HL1878] (GTX637627) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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GTX637627 ICC/IF Image
Galectin 3 antibody [HL1878] detects Galectin 3 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Galectin 3 stained by Galectin 3 antibody [HL1878] (GTX637627) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX637627 WB Image
Wild-type (WT) and Galectin 3 knockout (KO) HeLa cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Galectin 3 antibody [HL1878] (GTX637627) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637627 WB Image
Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Galectin 3 antibody [HL1878] (GTX637627) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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REFERENCE
There are currently no references for Galectin 3 antibody [HL1878] (GTX637627). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Galectin 3 antibody [HL1878] (GTX637627). Be the first to share your experience with this product.
![Anti-Galectin 3 antibody [HL1878] used in Western Blot (WB). GTX637627](/upload/website/prouct_img/small/GTX637627/GTX637627_44907_20221230_WB_TPM_watermark_23010400_694.webp "Anti-Galectin 3 antibody [HL1878] used in Western Blot (WB). GTX637627")
![Anti-Galectin 3 antibody [HL1878] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637627](/upload/website/prouct_img/small/GTX637627/GTX637627_T-44830_20221104_ICC_IF_22112219_738.webp "Anti-Galectin 3 antibody [HL1878] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637627")
![Anti-Galectin 3 antibody [HL1878] used in Western Blot (WB). GTX637627](/upload/website/prouct_img/small/GTX637627/GTX637627_T-44830_20221104_WB_KO_watermark_22110919_604.webp "Anti-Galectin 3 antibody [HL1878] used in Western Blot (WB). GTX637627")
![Anti-Galectin 3 antibody [HL1878] used in Western Blot (WB). GTX637627](/upload/website/prouct_img/small/GTX637627/GTX637627_T-44830_20221202_WB_M_22120719_374.webp "Anti-Galectin 3 antibody [HL1878] used in Western Blot (WB). GTX637627")
![Anti-Galectin 3 antibody [HL1878] used in Western Blot (WB). GTX637627](/upload/website/prouct_img/normal/GTX637627/GTX637627_44907_20221230_WB_TPM_watermark_23010400_694.webp "Anti-Galectin 3 antibody [HL1878] used in Western Blot (WB). GTX637627")
![Anti-Galectin 3 antibody [HL1878] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637627](/upload/website/prouct_img/normal/GTX637627/GTX637627_T-44830_20221104_ICC_IF_22112219_738.webp "Anti-Galectin 3 antibody [HL1878] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637627")
![Anti-Galectin 3 antibody [HL1878] used in Western Blot (WB). GTX637627](/upload/website/prouct_img/normal/GTX637627/GTX637627_T-44830_20221104_WB_KO_watermark_22110919_604.webp "Anti-Galectin 3 antibody [HL1878] used in Western Blot (WB). GTX637627")
![Anti-Galectin 3 antibody [HL1878] used in Western Blot (WB). GTX637627](/upload/website/prouct_img/normal/GTX637627/GTX637627_T-44830_20221202_WB_M_22120719_374.webp "Anti-Galectin 3 antibody [HL1878] used in Western Blot (WB). GTX637627")