GeneTex

  • Country / Location Selection

United States (US)

Galectin 3 antibody [HL2396]

Recombinant antibody KO/KD Orthogonal
Anti-Galectin 3 antibody [HL2396] used in Western Blot (WB). GTX638613
Anti-Galectin 3 antibody [HL2396] used in Western Blot (WB). GTX638613
Anti-Galectin 3 antibody [HL2396] used in IHC (Paraffin sections) (IHC-P). GTX638613
Anti-Galectin 3 antibody [HL2396] used in Western Blot (WB). GTX638613
Anti-Galectin 3 antibody [HL2396] used in IHC (Paraffin sections) (IHC-P). GTX638613
Anti-Galectin 3 antibody [HL2396] used in ELISA (ELISA). GTX638613
Anti-Galectin 3 antibody [HL2396] used in ELISA (ELISA). GTX638613
Anti-Galectin 3 antibody [HL2396] used in Western Blot (WB). GTX638613

Cat. No. GTX638613

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2396

Isotype

 IgG

Application

 WB, IHC-P, ELISA, Sandwich ELISA

Reactivity

 Human, Mouse, Rat, Dog
Package
100 μl ($399),
25 μl ($169)
See all Galectin 3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
IHC-P Assay dependent
ELISA Assay dependent
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture : GTX638614, Detection : GTX638613 or Capture : GTX638613, Detection : GTX638614
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragmemt of human Galectin 3

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

galectin 3 , CBP35 , GAL3 , GALBP , GALIG , L31 , LGALS2 , MAC2

Cellular Localization

Cytoplasm,Nucleus,Secreted

Background

This gene encodes a member of the galectin family of carbohydrate binding proteins. Members of this protein family have an affinity for beta-galactosides. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. This protein can self-associate through the N-terminal domain allowing it to bind to multivalent saccharide ligands. This protein localizes to the extracellular matrix, the cytoplasm and the nucleus. This protein plays a role in numerous cellular functions including apoptosis, innate immunity, cell adhesion and T-cell regulation. The protein exhibits antimicrobial activity against bacteria and fungi. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Oct 2014]

Database

Research Area

DATA IMAGES

Anti-Galectin 3 antibody [HL2396] used in Western Blot (WB). GTX638613

GTX638613 WB Image

KO/KD

Wild-type (WT) and Galectin 3 knockout (KO) HeLa cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Galectin 3 antibody [HL2396] (GTX638613) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Galectin 3 antibody [HL2396] used in Western Blot (WB). GTX638613

GTX638613 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Galectin 3 antibody [HL2396] (GTX638613) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Galectin 3 antibody [HL2396] used in IHC (Paraffin sections) (IHC-P). GTX638613

GTX638613 IHC-P Image

Galectin 3 antibody [HL2396] detects Galectin 3 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human kidney.
Galectin 3 stained by Galectin 3 antibody [HL2396] (GTX638613) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Galectin 3 antibody [HL2396] used in Western Blot (WB). GTX638613

GTX638613 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Galectin 3 antibody [HL2396] (GTX638613) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Galectin 3 antibody [HL2396] used in IHC (Paraffin sections) (IHC-P). GTX638613

GTX638613 IHC-P Image

Galectin 3 antibody [HL2396] detects Galectin 3 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissues.
Galectin 3 stained by Galectin 3 antibody [HL2396] (GTX638613) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Galectin 3 antibody [HL2396] used in ELISA (ELISA). GTX638613

GTX638613 ELISA Image

Sandwich ELISA detection of recombinant E.coli expressed, full-length Galectin 3 protein using antibodies as below.

Capture: Galectin 3 antibody [HL2397] (GTX638614) (5 μg/mL)
Detection: HRP-conjugated Galectin 3 antibody [HL2396] (GTX638613) (1 μg/mL)
Please notice that GTX638613 needs to be conjugated to HRP to function as the detection antibody when paired with GTX638614. Please contact us for custom HRP-conjugated antibody.

Anti-Galectin 3 antibody [HL2396] used in ELISA (ELISA). GTX638613

GTX638613 ELISA Image

Sandwich ELISA detection of recombinant E.coli expressed, full-length Galectin 3 protein using antibodies as below.

Capture: Galectin 3 antibody [HL2396] (GTX638613) (5 μg/mL)
Detection: HRP-conjugated Galectin 3 antibody [HL2397] (GTX638614) (1 μg/mL)
Please notice that GTX638614 needs to be conjugated to HRP to function as the detection antibody when paired with GTX638613. Please contact us for custom HRP-conjugated antibody.

Anti-Galectin 3 antibody [HL2396] used in Western Blot (WB). GTX638613

GTX638613 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Galectin 3 antibody [HL2396] (GTX638613) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

REFERENCE

There are currently no references for Galectin 3 antibody [HL2396] (GTX638613). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for Galectin 3 antibody [HL2396] (GTX638613). Be the first to share your experience with this product.
Submit a Review
Package List Price ($)
$ 399
$ 169
Custom Conjugations