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Galectin 3 antibody

Anti-Galectin 3 antibody used in Western Blot (WB). GTX113486
Anti-Galectin 3 antibody used in Western Blot (WB). GTX113486
Anti-Galectin 3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113486
Anti-Galectin 3 antibody used in IHC (Paraffin sections) (IHC-P). GTX113486
Anti-Galectin 3 antibody used in Western Blot (WB). GTX113486
Anti-Galectin 3 antibody used in Western Blot (WB). GTX113486
Anti-Galectin 3 antibody used in Western Blot (WB). GTX113486

Cat No. GTX113486

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Rat, Dog
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

Positive Control

HeLa , A549 , H1299 , HCT116 , MCF-7 , Recombinant LGALS3 protein , rat colon

Predict Reactivity

Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Galectin3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

galectin 3,CBP35,GAL3,GALBP,GALIG,L31,LGALS2,MAC2

Cellular Localization

Nucleus , Cytoplasm , Secreted

Background

This gene encodes a member of the galectin family of carbohydrate binding proteins. Members of this protein family have an affinity for beta-galactosides. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. This protein can self-associate through the N-terminal domain allowing it to bind to multivalent saccharide ligands. This protein localizes to the extracellular matrix, the cytoplasm and the nucleus. This protein plays a role in numerous cellular functions including apoptosis, innate immunity, cell adhesion and T-cell regulation. Alternate splicing results in multiple transcript variants.

Database

Research Area

DATA IMAGES
Anti-Galectin 3 antibody used in Western Blot (WB). GTX113486

GTX113486 WB Image

Wild-type (WT) and Galectin 3 knockout (KO) HeLa cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Galectin 3 antibody (GTX113486) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Galectin 3 antibody used in Western Blot (WB). GTX113486

GTX113486 WB Image

Sample (30 ug of whole cell lysate)
A: Non-transfected 293T lysates
B: LGALS3 transfected 293T lysates
12% SDS PAGE
GTX113486 diluted at 1:10000

Anti-Galectin 3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113486

GTX113486 ICC/IF Image

Galectin3 antibody detects Galectin3 protein at cytoplasm by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min.
Green: Galectin3 protein stained by Galectin3 antibody (GTX113486) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-Galectin 3 antibody used in IHC (Paraffin sections) (IHC-P). GTX113486

GTX113486 IHC-P Image

Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using Galectin3(GTX113486) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Galectin 3 antibody used in Western Blot (WB). GTX113486

GTX113486 WB Image

Galectin3 antibody detects Galectin3 protein by western blot analysis.
A. 50 μg rat colon lysate/extract
12 % SDS-PAGE
Galectin3 antibody (GTX113486) dilution: 1:10000

Anti-Galectin 3 antibody used in Western Blot (WB). GTX113486

GTX113486 WB Image

Whole cell extract (30 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with Galectin 3 antibody (GTX113486) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Galectin 3 antibody used in Western Blot (WB). GTX113486

GTX113486 WB Image

Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Galectin 3 antibody (GTX113486) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf