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Galectin 3 antibody

KO/KD Orthogonal Overexpression
Anti-Galectin 3 antibody used in Western Blot (WB). GTX113486
Anti-Galectin 3 antibody used in IHC (Paraffin sections) (IHC-P). GTX113486
Anti-Galectin 3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113486
Anti-Galectin 3 antibody used in Western Blot (WB). GTX113486
Anti-Galectin 3 antibody used in Western Blot (WB). GTX113486
Anti-Galectin 3 antibody used in ELISA (ELISA). GTX113486
Anti-Galectin 3 antibody used in Western Blot (WB). GTX113486
Anti-Galectin 3 antibody used in Western Blot (WB). GTX113486

Cat. No. GTX113486

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, ELISA, Sandwich ELISA

Reactivity

 Human, Rat, Dog
Package
100 μl ($399),
25 μl ($169)
See all Galectin 3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
ELISA Assay dependent
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture : GTX635156, Detection: GTX113486
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

Positive Control

LGALS3 transfected 293T , rat colon , HeLa , AsPC-1

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.85 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Galectin 3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11164060

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

galectin 3 , CBP35 , GAL3 , GALBP , GALIG , L31 , LGALS2 , MAC2

Cellular Localization

Nucleus , Cytoplasm , Secreted

Background

This gene encodes a member of the galectin family of carbohydrate binding proteins. Members of this protein family have an affinity for beta-galactosides. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. This protein can self-associate through the N-terminal domain allowing it to bind to multivalent saccharide ligands. This protein localizes to the extracellular matrix, the cytoplasm and the nucleus. This protein plays a role in numerous cellular functions including apoptosis, innate immunity, cell adhesion and T-cell regulation. Alternate splicing results in multiple transcript variants.

Database

Research Area

DATA IMAGES

Anti-Galectin 3 antibody used in Western Blot (WB). GTX113486

GTX113486 WB Image

KO/KD

Wild-type (WT) and Galectin 3 knockout (KO) HeLa cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Galectin 3 antibody (GTX113486) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Galectin 3 antibody used in IHC (Paraffin sections) (IHC-P). GTX113486

GTX113486 IHC-P Image

Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using Galectin3(GTX113486) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Galectin 3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113486

GTX113486 ICC/IF Image

Galectin3 antibody detects Galectin3 protein at cytoplasm by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min.
Green: Galectin3 protein stained by Galectin3 antibody (GTX113486) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-Galectin 3 antibody used in Western Blot (WB). GTX113486

GTX113486 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Galectin 3 antibody (GTX113486) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Galectin 3 antibody used in Western Blot (WB). GTX113486

GTX113486 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Galectin 3 antibody (GTX113486) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-Galectin 3 antibody used in ELISA (ELISA). GTX113486

GTX113486 ELISA Image

Sandwich ELISA detection of recombinant full-length Galectin 3 protein using GTX635156 as capture antibody at concentration of 5 μg/mL and GTX113486 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-Galectin 3 antibody used in Western Blot (WB). GTX113486

GTX113486 WB Image

Overexpression

Sample (30 ug of whole cell lysate)
A: Non-transfected 293T lysates
B: LGALS3 transfected 293T lysates
12% SDS PAGE
GTX113486 diluted at 1:10000

Anti-Galectin 3 antibody used in Western Blot (WB). GTX113486

GTX113486 WB Image

Galectin3 antibody detects Galectin3 protein by western blot analysis.
A. 50 μg rat colon lysate/extract
12 % SDS-PAGE
Galectin3 antibody (GTX113486) dilution: 1:10000

REFERENCE

Application Reference

Liu X et al. J Cardiovasc Pharmacol 2019; 74 (1) : 13-19 Ticagrelor Reduces Ischemia-Reperfusion Injury Through the NF-κB-Dependent Pathway in Rats.
Application : WB
Reactivity : Rat
Honig E et al. J Cell Sci 2018; (Epub) Galectin-3 modulates the polarized surface delivery of β1-integrin in epithelial cells.
Application : WB, ICC/IF
Reactivity : Dog
Liu X et al. Br J Clin Pharmacol 2018; (Epub) Ticagrelor attenuates myocardial ischemia-reperfusion injury possibly through downregulating galectin-3 expression in the infarct area of rats.
Application : WB
Reactivity : Rat
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169