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Galectin 3 antibody

Anti-Galectin 3 antibody used in Western Blot (WB). GTX113486
Anti-Galectin 3 antibody used in ELISA (ELISA). GTX113486
Anti-Galectin 3 antibody used in Western Blot (WB). GTX113486
Anti-Galectin 3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113486
Anti-Galectin 3 antibody used in IHC (Paraffin sections) (IHC-P). GTX113486
Anti-Galectin 3 antibody used in Western Blot (WB). GTX113486
Anti-Galectin 3 antibody used in Western Blot (WB). GTX113486
Anti-Galectin 3 antibody used in Western Blot (WB). GTX113486

Cat No. GTX113486

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, ELISA

Reactivity

Human, Rat, Dog
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
ELISA Assay dependent
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

Positive Control

HeLa , A549 , H1299 , HCT116 , MCF-7 , Recombinant LGALS3 protein , rat colon

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Galectin3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

galectin 3 , CBP35 , GAL3 , GALBP , GALIG , L31 , LGALS2 , MAC2

Cellular Localization

Nucleus , Cytoplasm , Secreted

Background

This gene encodes a member of the galectin family of carbohydrate binding proteins. Members of this protein family have an affinity for beta-galactosides. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. This protein can self-associate through the N-terminal domain allowing it to bind to multivalent saccharide ligands. This protein localizes to the extracellular matrix, the cytoplasm and the nucleus. This protein plays a role in numerous cellular functions including apoptosis, innate immunity, cell adhesion and T-cell regulation. Alternate splicing results in multiple transcript variants.

Database

Research Area

DATA IMAGES

Anti-Galectin 3 antibody used in Western Blot (WB). GTX113486

GTX113486 WB Image

Wild-type (WT) and Galectin 3 knockout (KO) HeLa cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Galectin 3 antibody (GTX113486) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Galectin 3 antibody used in ELISA (ELISA). GTX113486

GTX113486 ELISA Image

Sandwich ELISA detection of recombinant full-length Galectin 3 protein using GTX635156 as capture antibody at concentration of 5 μg/mL and GTX113486 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-Galectin 3 antibody used in Western Blot (WB). GTX113486

GTX113486 WB Image

Sample (30 ug of whole cell lysate)
A: Non-transfected 293T lysates
B: LGALS3 transfected 293T lysates
12% SDS PAGE
GTX113486 diluted at 1:10000

Anti-Galectin 3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113486

GTX113486 ICC/IF Image

Galectin3 antibody detects Galectin3 protein at cytoplasm by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min.
Green: Galectin3 protein stained by Galectin3 antibody (GTX113486) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-Galectin 3 antibody used in IHC (Paraffin sections) (IHC-P). GTX113486

GTX113486 IHC-P Image

Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using Galectin3(GTX113486) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Galectin 3 antibody used in Western Blot (WB). GTX113486

GTX113486 WB Image

Galectin3 antibody detects Galectin3 protein by western blot analysis.
A. 50 μg rat colon lysate/extract
12 % SDS-PAGE
Galectin3 antibody (GTX113486) dilution: 1:10000

Anti-Galectin 3 antibody used in Western Blot (WB). GTX113486

GTX113486 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Galectin 3 antibody (GTX113486) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Galectin 3 antibody used in Western Blot (WB). GTX113486

GTX113486 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Galectin 3 antibody (GTX113486) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169