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Galectin 3 antibody

Anti-Galectin 3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX35218
Anti-Galectin 3 antibody used in Western Blot (WB). GTX35218
Anti-Galectin 3 antibody used in Western Blot (WB). GTX35218
Anti-Galectin 3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX35218
Anti-Galectin 3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX35218

Cat No. GTX35218

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human, Mouse, Rat
Package
100 μl ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 50% glycerol, 0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-1-250 of human LGALS3 (NP_002297.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

galectin 3 , CBP35 , GAL3 , GALBP , GALIG , L31 , LGALS2 , MAC2

Cellular Localization

Cytoplasm,Nucleus,Secreted

Background

This gene encodes a member of the galectin family of carbohydrate binding proteins. Members of this protein family have an affinity for beta-galactosides. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. This protein can self-associate through the N-terminal domain allowing it to bind to multivalent saccharide ligands. This protein localizes to the extracellular matrix, the cytoplasm and the nucleus. This protein plays a role in numerous cellular functions including apoptosis, innate immunity, cell adhesion and T-cell regulation. The protein exhibits antimicrobial activity against bacteria and fungi. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Oct 2014]

Database

Research Area

DATA IMAGES

Anti-Galectin 3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX35218

GTX35218 ICC/IF Image

ICC/IF analysis of L929 cells using GTX35218 Galectin 3 antibody.
Blue : DAPI
Dilution : 1:100

Anti-Galectin 3 antibody used in Western Blot (WB). GTX35218

GTX35218 WB Image

WB analysis of various sample lysates using GTX35218 Galectin 3 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-Galectin 3 antibody used in Western Blot (WB). GTX35218

GTX35218 WB Image

WB analysis of normal (control) and knockout (KO) HeLa cell lysate using GTX35218 Galectin 3 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-Galectin 3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX35218

GTX35218 ICC/IF Image

ICC/IF analysis of C6 cells using GTX35218 Galectin 3 antibody.
Blue : DAPI
Dilution : 1:100

Anti-Galectin 3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX35218

GTX35218 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX35218 Galectin 3 antibody.
Blue : DAPI
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf