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Galectin 4 antibody

Orthogonal Overexpression
Anti-Galectin 4 antibody used in Western Blot (WB). GTX114527
Anti-Galectin 4 antibody used in IHC (Paraffin sections) (IHC-P). GTX114527
Anti-Galectin 4 antibody used in Western Blot (WB). GTX114527

Cat. No. GTX114527

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IP

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Galectin 4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

LGALS4 transfected 293T

Predict Reactivity

Rabbit(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.02 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Galectin 4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885342

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

galectin 4 , GAL4 , L36LBP

Background

The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. The expression of this gene is restricted to small intestine, colon, and rectum, and it is underexpressed in colorectal cancer. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Galectin 4 antibody used in Western Blot (WB). GTX114527

GTX114527 WB Image

Overexpression

Sample (30 ug of whole cell lysate)
A: Non-transfected 293T lysates
B: LGALS4 transfected 293T lysate
10% SDS PAGE
GTX114527 diluted at 1:5000

Anti-Galectin 4 antibody used in IHC (Paraffin sections) (IHC-P). GTX114527

GTX114527 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using Galectin4(GTX114527) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Galectin 4 antibody used in Western Blot (WB). GTX114527

GTX114527 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Galectin 4 antibody (GTX114527) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

REFERENCE

Application Reference

Tsai CH et al. Cancer Res 2016; 76 (19) : 5756-5767 Metastatic Progression of Prostate Cancer Is Mediated by Autonomous Binding of Galectin-4-O-Glycan to Cancer Cells.
Application : WB, IHC-P, IP
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169