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Galectin 4 antibody

Anti-Galectin 4 antibody used in Western Blot (WB). GTX125898
Anti-Galectin 4 antibody used in Western Blot (WB). GTX125898
Anti-Galectin 4 antibody used in Western Blot (WB). GTX125898
Anti-Galectin 4 antibody used in IHC (Paraffin sections) (IHC-P). GTX125898
Anti-Galectin 4 antibody used in Western Blot (WB). GTX125898

Cat No. GTX125898

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

HCT116 , LGALS4 transfected 293T lysate , Mouse colon , rat colon

Predict Reactivity

Rabbit, Bovine, Dog, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of Human Galectin 4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

galectin 4 , GAL4 , L36LBP

Background

The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. The expression of this gene is restricted to small intestine, colon, and rectum, and it is underexpressed in colorectal cancer. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Galectin 4 antibody used in Western Blot (WB). GTX125898

GTX125898 WB Image

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Galectin4 antibody (GTX125898) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Galectin 4 antibody used in Western Blot (WB). GTX125898

GTX125898 WB Image

Sample (30 μg of whole cell lysate)
A: Non-transfected 293T lysates
B: LGALS4 transfected 293T lysate
10% SDS PAGE
GTX125898 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Galectin 4 antibody used in Western Blot (WB). GTX125898

GTX125898 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Galectin4 antibody (GTX125898) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Galectin 4 antibody used in IHC (Paraffin sections) (IHC-P). GTX125898

GTX125898 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using Galectin4(GTX125898) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Galectin 4 antibody used in Western Blot (WB). GTX125898

GTX125898 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Galectin4 antibody (GTX125898) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169