Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Galectin 4 antibody (GTX125898) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Galectin4 antibody (GTX125898) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample (30 μg of whole cell lysate)
A: Non-transfected 293T lysates
B: LGALS4 transfected 293T lysate
10% SDS PAGE
GTX125898 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Galectin4 antibody (GTX125898) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Galectin4 antibody (GTX125898) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using Galectin4(GTX125898) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min