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Gapdh antibody

Anti-Gapdh antibody used in IHC (Paraffin sections) (IHC-P). GTX124502
Anti-Gapdh antibody used in Western Blot (WB). GTX124502
Anti-Gapdh antibody used in Western Blot (WB). GTX124502
Anti-Gapdh antibody used in Western Blot (WB). GTX124502
Anti-Gapdh antibody used in Western Blot (WB). GTX124502
Anti-Gapdh antibody used in Western Blot (WB). GTX124502
Anti-Gapdh antibody used in IHC (Paraffin sections) (IHC-P). GTX124502
Anti-Gapdh antibody used in IHC (Paraffin sections) (IHC-P). GTX124502
Anti-Gapdh antibody used in Western Blot (WB). GTX124502
Anti-Gapdh antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124502
Anti-Gapdh antibody used in IHC (Paraffin sections) (IHC-P). GTX124502
Anti-Gapdh antibody used in Western Blot (WB). GTX124502
Anti-Gapdh antibody used in Western Blot (WB). GTX124502
Anti-Gapdh antibody used in Western Blot (WB). GTX124502

Cat. No. GTX124502

Reference 6

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat, Zebrafish, Drosophila, Cat, Dog, E. coli
Package
100 μl ($399),
25 μl ($169)
See all zebrafish Gapdh products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Predict Reactivity

Human (100%)| Mouse (94%)| Rat (94%)| Zebrafish (86%)| Rabbit (94%)| Sheep (94%)| Bovine (94%)| Cat (96%)| Dog (95%)| Chicken (92%)| Guinea pig (94%)| Pig (95%)| Xenopus tropicalis (85%)| Rhesus Monkey (99%)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.11 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Zebrafish

Immunogen

The immunogen used to generate this antibody corresponds to zebrafish Gapdh

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11177426

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glyceraldehyde-3-phosphate dehydrogenase , bb02e05 , cb609 , gapd , mg:bb02e05 , wu:fb33a10 , wu:ft80f05

Database

Research Area

DATA IMAGES

Anti-Gapdh antibody used in IHC (Paraffin sections) (IHC-P). GTX124502

GTX124502 IHC-P Image

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using gapdh (GTX124502) antibody at 1:300 dilution.

Anti-Gapdh antibody used in Western Blot (WB). GTX124502

GTX124502 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Gapdh antibody (GTX124502) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Gapdh antibody used in Western Blot (WB). GTX124502

GTX124502 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Gapdh antibody (GTX124502) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Gapdh antibody used in Western Blot (WB). GTX124502

GTX124502 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Gapdh antibody (GTX124502) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Gapdh antibody used in Western Blot (WB). GTX124502

GTX124502 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Gapdh antibody (GTX124502) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Gapdh antibody used in Western Blot (WB). GTX124502

GTX124502 WB Image

Whole zebrafish extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Gapdh antibody (GTX124502) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Gapdh antibody used in IHC (Paraffin sections) (IHC-P). GTX124502

GTX124502 IHC-P Image

Gapdh antibody detects Gapdh protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human human gastric cancer.
Gapdh stained by Gapdh antibody (GTX124502) diluted at 1:100.
Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 8 min

Anti-Gapdh antibody used in IHC (Paraffin sections) (IHC-P). GTX124502

GTX124502 IHC-P Image

Gapdh antibody detects Gapdh protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
Gapdh stained by Gapdh antibody (GTX124502) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Gapdh antibody used in Western Blot (WB). GTX124502

GTX124502 WB Image

Drosophila extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Gapdh antibody (GTX124502) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Gapdh antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124502

GTX124502 ICC/IF Image

Gapdh antibody detects Gapdh protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Gapdh stained by Gapdh antibody (GTX124502) diluted at 1:6000.

Anti-Gapdh antibody used in IHC (Paraffin sections) (IHC-P). GTX124502

GTX124502 IHC-P Image

Gapdh antibody detects Gapdh protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat kidney.
Gapdh stained by Gapdh antibody (GTX124502) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Gapdh antibody used in Western Blot (WB). GTX124502

GTX124502 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Gapdh antibody (GTX124502) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Gapdh antibody used in Western Blot (WB). GTX124502

GTX124502 WB Image

The data was published in the 2022 in Anal Cell Pathol (Amst). PMID: 35498155

Anti-Gapdh antibody used in Western Blot (WB). GTX124502

GTX124502 WB Image

The data was published in the 2022 in Anal Cell Pathol (Amst). PMID: 35498155

REFERENCE

Application Reference

Xuan DTM et al. Int J Med Sci 2023; 20 (1) : 35-49 Glutamine synthetase regulates the immune microenvironment and cancer development through the inflammatory pathway.
Zhuo Wang et al. Anal Cell Pathol (Amst) 2022; 2022 : 9675466 FOXA1 Leads to Aberrant Expression of SIX4 Affecting Cervical Cancer Cell Growth and Chemoresistance.
Application : WB
Reactivity : Human
Zhu X et al. FEBS Open Bio 2021; (Epub) VDAC1 oligomerization may enhance DDP-induced hepatocyte apoptosis by exacerbating oxidative stress and mitochondrial DNA damage.
Application : WB
Reactivity : Human
Mei J et al. Oncol Lett 2020; 20 (4) : 7 OIP5-AS1 modulates epigenetic regulator HDAC7 to enhance non-small cell lung cancer metastasis via miR-140-5p.
Lin W et al. Sci Total Environ 2019; 702 : 134969 Waterborne microcystin-LR exposure induced chronic inflammatory response via MyD88-dependent toll-like receptor signaling pathway in male zebrafish.
Application : WB
Reactivity : Zebrafish
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169