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Gasdermin D antibody [6D11]

Anti-Gasdermin D antibody [6D11] used in Flow cytometry (FACS). GTX04538
Anti-Gasdermin D antibody [6D11] used in IHC (Paraffin sections) (IHC-P). GTX04538
Anti-Gasdermin D antibody [6D11] used in Western Blot (WB). GTX04538
Anti-Gasdermin D antibody [6D11] used in IHC (Paraffin sections) (IHC-P). GTX04538
Anti-Gasdermin D antibody [6D11] used in IHC (Paraffin sections) (IHC-P). GTX04538
Anti-Gasdermin D antibody [6D11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04538

Cat. No. GTX04538

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 6D11

Isotype

 IgG2b

Application

 WB, ICC/IF, IHC-P, FACS, ELISA

Reactivity

 Human
Package
100 μg ($469)
See all Gasdermin D products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.25-0.5 μg/mL
ICC/IF 5 μg/mL
IHC-P 2-5 μg/mL
FACS 1-3 μg/1x10⁶ cells
ELISA Assay dependent
Not tested in other applications.

Calculated MW

53 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄

Preservative

No preservatives

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

E.coli-derived human GSDMD recombinant protein (Position: M1-H484).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

gasdermin D , DF5L , DFNA5L , FKSG10 , GSDMDC1

Cellular Localization

Secreted

Background

Gasdermin D is a member of the gasdermin family. Members of this family appear to play a role in regulation of epithelial proliferation. Gasdermin D has been suggested to act as a tumor suppressor. Alternatively spliced transcript variants have been described. [provided by RefSeq, Oct 2009]

Database

Research Area

DATA IMAGES

Anti-Gasdermin D antibody [6D11] used in Flow cytometry (FACS). GTX04538

GTX04538 FACS Image

FACS analysis of Jurkat cells using GTX04538 Gasdermin D antibody [6D11].
Blue : Primary antibody
Green : Isotype control
Red : Unlabelled sample
Dilution : 1 μg/1x10⁶ cells

Anti-Gasdermin D antibody [6D11] used in IHC (Paraffin sections) (IHC-P). GTX04538

GTX04538 IHC-P Image

IHC-P analysis of human esophageal squamous carcinoma tissue using GTX04538 Gasdermin D antibody [6D11].
Antigen retrieval : Heat mediated EDTA buffer (pH 8.0)
Dilution : 2 μg/mL

Anti-Gasdermin D antibody [6D11] used in Western Blot (WB). GTX04538

GTX04538 WB Image

WB analysis of various samples using GTX04538 Gasdermin D antibody [6D11].
Loading : 30 μg
Dilution : 0.5 μg/mL

Anti-Gasdermin D antibody [6D11] used in IHC (Paraffin sections) (IHC-P). GTX04538

GTX04538 IHC-P Image

IHC-P analysis of human cervical cancern tissue using GTX04538 Gasdermin D antibody [6D11].
Antigen retrieval : Heat mediated EDTA buffer (pH 8.0)
Dilution : 2 μg/mL

Anti-Gasdermin D antibody [6D11] used in IHC (Paraffin sections) (IHC-P). GTX04538

GTX04538 IHC-P Image

IHC-P analysis of human cervical intraepithelial neoplasia tissue using GTX04538 Gasdermin D antibody [6D11].
Antigen retrieval : Heat mediated EDTA buffer (pH 8.0)
Dilution : 2 μg/mL

Anti-Gasdermin D antibody [6D11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04538

GTX04538 ICC/IF Image

ICC/IF analysis of PC-3 cells using GTX04538 Gasdermin D antibody [6D11].
Antigen retrieval : IHC enzyme antigen retrieval reagent
Green : Primary antibody
Blue : DAPI
Dilution : 5 μg/mL

REFERENCE

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Package List Price ($)
$ 469