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Gasdermin D antibody

Orthogonal
Anti-Gasdermin D antibody used in Western Blot (WB). GTX04690
Anti-Gasdermin D antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04690
Anti-Gasdermin D antibody used in Western Blot (WB). GTX04690

Cat. No. GTX04690

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all Gasdermin D products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
ICC/IF 1:50-1:200
IHC-P 1:50-1:200
Not tested in other applications.

Calculated MW

53 kDa. ( Note )

Predict Reactivity

Pig, Horse(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS pH 7.4, 150 mM NaCl, 50% glycerol

Preservative

0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human GSDMD(Accession P57764), corresponding to amino acid residues W50-G100.

Purification

Purified by antigen-affinity chromatography
From serum

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

gasdermin D , DF5L , DFNA5L , FKSG10 , GSDMDC1

Cellular Localization

Secreted

Background

Gasdermin D is a member of the gasdermin family. Members of this family appear to play a role in regulation of epithelial proliferation. Gasdermin D has been suggested to act as a tumor suppressor. Alternatively spliced transcript variants have been described. [provided by RefSeq, Oct 2009]

Database

Research Area

DATA IMAGES

Anti-Gasdermin D antibody used in Western Blot (WB). GTX04690

GTX04690 WB Image

WB analysis of B16F10 whole cell lysate using GTX04690 Gasdermin D antibody. The lane on the left was treated with blocking peptide.

Anti-Gasdermin D antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04690

GTX04690 ICC/IF Image

ICC/IF analysis of PFA-fixed HeLa cells with LPS-treatment using GTX04690 Gasdermin D antibody.
Red : Primary antibody
Green : Beta tubulin
Blue : DAPI
Permeabilization : 0.1% Triton X-100
Dilution : 1:200

Anti-Gasdermin D antibody used in Western Blot (WB). GTX04690

GTX04690 WB Image

Orthogonal

WB analysis of various samples using GTX04690 Gasdermin D antibody.
Lane 1 : TPA + LPS treated C6 whole cell lysate with blocking peptide
Lane 2 : TPA-treated C6 whole cell lysate
Lane 3 : TPA-treated HepG2 whole cell lysate
Lane 4 : TPA + LPS treated C6 whole cell lysate
Lane 5 : TPA + LPS treated HepG2 whole cell lysate

REFERENCE

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REVIEW

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Package List Price ($)
$ 399