Detection of Gcn5 protein in crude lysate of S. cerevisiae strain BY4741 by western blotting with anti-Gcn5 antibody. Anti-Gcn5 antibody was used at 1/500 dilution and 2nd antibody, goat anti-rabbit IgG antibody conjugated with HRP, was used at 1/5,000 dilution. Signal enhancer was used. Numbers on the left are positions of protein bands in kDa. Molecular mass of Gcn5 is 51 kDa.