GeneTex
United States (US)

Gelsolin antibody [C2C3], C-term

KO/KD
GTX101185_40093_20160526_WB_shRNA_watermark_19011506_153.jpg
GTX101185_IHC_19011506_658.jpg
GTX101185_WB_19011506_430.jpg
GTX101185_40093_20150910_WB_H_plasma_19011506_778.jpg
GTX101185_40093_20160317_WB_R_plasma_19011506_541.jpg
GTX101185_40093_IFA_19011506_690.jpg
GTX101185_40093_20160317_WB_M_plasma_19011506_587.jpg

Cat No. GTX101185

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

86 kDa. ( Note )

Positive Control

H1299 , HeLa , human plasma , rat plasma , mouse plasma

Predict Reactivity

Sheep, Bovine, Chicken(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.27mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Gelsolin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Gelsolin,Adf,Agel,Gsn

Cellular Localization

Isoform 2: Cytoplasm , cytoskeleton , Isoform 1: Secreted

Background

The protein encoded by this gene binds to the "plus" ends of actin monomers and filaments to prevent monomer exchange. The encoded calcium-regulated protein functions in both assembly and disassembly of actin filaments. Defects in this gene are a cause of familial amyloidosis Finnish type (FAF). Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101185_40093_20160526_WB_shRNA_watermark_19011506_153.jpg

GTX101185 WB Image

KO/KD

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Gelsolin antibody [C2C3], C-term (GTX101185) diluted at 1:500.

GTX101185_IHC_19011506_658.jpg

GTX101185 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using Gelsolin(GTX101185) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101185_WB_19011506_430.jpg

GTX101185 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
B: Hela
7.5% SDS PAGE
GTX101185 diluted at 1:1000

GTX101185_40093_20150910_WB_H_plasma_19011506_778.jpg

GTX101185 WB Image

Gelsolin antibody detects Gelsolin protein by western blot analysis. Human tissue extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Gelsolin antibody (GTX101185) diluted at 1:1000.

GTX101185_40093_20160317_WB_R_plasma_19011506_541.jpg

GTX101185 WB Image

Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Gelsolin antibody [C2C3], C-term (GTX101185) diluted at 1:500.

GTX101185_40093_IFA_19011506_690.jpg

GTX101185 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using Gelsolin(GTX101185) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

GTX101185_40093_20160317_WB_M_plasma_19011506_587.jpg

GTX101185 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Gelsolin antibody [C2C3], C-term (GTX101185) diluted at 1:500.

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf