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Gelsolin antibody [C2C3], C-term

Anti-Gelsolin antibody [C2C3], C-term used in Western Blot (WB). GTX101185
Anti-Gelsolin antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101185
Anti-Gelsolin antibody [C2C3], C-term used in Western Blot (WB). GTX101185
Anti-Gelsolin antibody [C2C3], C-term used in Western Blot (WB). GTX101185
Anti-Gelsolin antibody [C2C3], C-term used in Western Blot (WB). GTX101185
Anti-Gelsolin antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101185
Anti-Gelsolin antibody [C2C3], C-term used in Western Blot (WB). GTX101185

Cat No. GTX101185

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

86 kDa. ( Note )

Positive Control

H1299 , HeLa , human plasma , rat plasma , mouse plasma

Predict Reactivity

Sheep, Bovine, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.27 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Gelsolin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

gelsolin , ADF , AGEL

Cellular Localization

Isoform 2: Cytoplasm , cytoskeleton , Isoform 1: Secreted

Background

The protein encoded by this gene binds to the "plus" ends of actin monomers and filaments to prevent monomer exchange. The encoded calcium-regulated protein functions in both assembly and disassembly of actin filaments. Defects in this gene are a cause of familial amyloidosis Finnish type (FAF). Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Gelsolin antibody [C2C3], C-term used in Western Blot (WB). GTX101185

GTX101185 WB Image

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Gelsolin antibody [C2C3], C-term (GTX101185) diluted at 1:500.

Anti-Gelsolin antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101185

GTX101185 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using Gelsolin(GTX101185) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Gelsolin antibody [C2C3], C-term used in Western Blot (WB). GTX101185

GTX101185 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
B: Hela
7.5% SDS PAGE
GTX101185 diluted at 1:1000

Anti-Gelsolin antibody [C2C3], C-term used in Western Blot (WB). GTX101185

GTX101185 WB Image

Gelsolin antibody detects Gelsolin protein by western blot analysis. Human tissue extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Gelsolin antibody (GTX101185) diluted at 1:1000.

Anti-Gelsolin antibody [C2C3], C-term used in Western Blot (WB). GTX101185

GTX101185 WB Image

Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Gelsolin antibody [C2C3], C-term (GTX101185) diluted at 1:500.

Anti-Gelsolin antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101185

GTX101185 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using Gelsolin(GTX101185) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-Gelsolin antibody [C2C3], C-term used in Western Blot (WB). GTX101185

GTX101185 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Gelsolin antibody [C2C3], C-term (GTX101185) diluted at 1:500.

REFERENCE

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169