Gelsolin antibody [GT1656]

Name: Gelsolin antibody [GT1656]
Brand: GeneTex
SKU: GTX633690
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-Gelsolin antibody [GT1656] used in Western Blot (WB). GTX633690

Anti-Gelsolin antibody [GT1656] used in IHC (Paraffin sections) (IHC-P). GTX633690

Cat. No. GTX633690

Host	Mouse
Clonality	Monoclonal
Clone Name	GT1656
Isotype	IgG1
Application	WB, IHC-P
Reactivity	Human, Mouse, Rat
Package	100 μl ($399), 25 μl ($169)

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Datasheet
Document

View product citations for antibody GTX633690 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

86 kDa. ( Note )

Positive Control

HeLa , mouse plasma , rat plasma

Predict Reactivity

Sheep, Bovine, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Gelsolin. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2885052

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

gelsolin , ADF , AGEL

Cellular Localization

Isoform 2: Cytoplasm , cytoskeleton , Isoform 1: Secreted

Background

The protein encoded by this gene binds to the "plus" ends of actin monomers and filaments to prevent monomer exchange. The encoded calcium-regulated protein functions in both assembly and disassembly of actin filaments. Defects in this gene are a cause of familial amyloidosis Finnish type (FAF). Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq]

Database

Gene ID: 2934 GSN
UniProt: P06396 GSN

Research Area

DATA IMAGES

GTX633690 WB Image

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Gelsolin antibody [GT1656] (GTX633690) diluted at 1:500.

GTX633690 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Gelsolin antibody [GT1656] (GTX633690) diluted at 1:1000.

GTX633690 IHC-P Image

Gelsolin antibody [GT1656] detects Gelsolin protein at cytoplasm in rat muscle by immunohistochemical analysis.
Sample: Paraffin-embedded rat muscle.
Gelsolin antibody [GT1656] (GTX633690) diluted at 1:200.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX633690 IHC-P Image

Gelsolin antibody [GT1656] detects secreted Gelsolin protein in rat duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded rat duodenum.
Gelsolin antibody [GT1656] (GTX633690) diluted at 1:200.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX633690 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Gelsolin antibody [GT1656] (GTX633690) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX633690 WB Image

Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Gelsolin antibody [GT1656] (GTX633690) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

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REVIEW