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Gelsolin antibody [HL1930]

Recombinant antibody KO/KD
Anti-Gelsolin antibody [HL1930] used in Western Blot (WB). GTX637767
Anti-Gelsolin antibody [HL1930] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637767
Anti-Gelsolin antibody [HL1930] used in Western Blot (WB). GTX637767
Anti-Gelsolin antibody [HL1930] used in Western Blot (WB). GTX637767

Cat. No. GTX637767

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1930

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Gelsolin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

86 kDa. ( Note )

Predict Reactivity

Sheep, Bovine, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human Gelsolin

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

gelsolin , ADF , AGEL

Background

The protein encoded by this gene binds to the "plus" ends of actin monomers and filaments to prevent monomer exchange. The encoded calcium-regulated protein functions in both assembly and disassembly of actin filaments. Defects in this gene are a cause of familial amyloidosis Finnish type (FAF). Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Gelsolin antibody [HL1930] used in Western Blot (WB). GTX637767

GTX637767 WB Image

KO/KD

Non-transfected (–) and transfected (+) HeLa whole cell extract (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Gelsolin antibody [HL1930] (GTX637767) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Gelsolin antibody [HL1930] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637767

GTX637767 ICC/IF Image

Gelsolin antibody [HL1930] detects Gelsolin protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Gelsolin stained by Gelsolin antibody [HL1930] (GTX637767) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Gelsolin antibody [HL1930] used in Western Blot (WB). GTX637767

GTX637767 WB Image

Mouse plasma and rat plasma (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Gelsolin antibody [HL1930] (GTX637767) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Gelsolin antibody [HL1930] used in Western Blot (WB). GTX637767

GTX637767 WB Image

Human plasma (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Gelsolin antibody [HL1930] (GTX637767) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations