GeneTex
  • Country / Location Selection

United States (US)

Gelsolin antibody

Anti-Gelsolin antibody used in Western Blot (WB). GTX114078
Anti-Gelsolin antibody used in Western Blot (WB). GTX114078
Anti-Gelsolin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114078
Anti-Gelsolin antibody used in IHC (Paraffin sections) (IHC-P). GTX114078
Anti-Gelsolin antibody used in Western Blot (WB). GTX114078
Anti-Gelsolin antibody used in Western Blot (WB). GTX114078
Anti-Gelsolin antibody used in Western Blot (WB). GTX114078
Anti-Gelsolin antibody used in IHC (Paraffin sections) (IHC-P). GTX114078

Cat No. GTX114078

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

86 kDa. ( Note )

Positive Control

H1299 , HeLa , human plasma , mouse plasma , rat plasma

Predict Reactivity

Sheep, Bovine, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.13 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Gelsolin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037068

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

gelsolin , ADF , AGEL

Cellular Localization

Isoform 2: Cytoplasm , cytoskeleton , Isoform 1: Secreted

Background

The protein encoded by this gene binds to the "plus" ends of actin monomers and filaments to prevent monomer exchange. The encoded calcium-regulated protein functions in both assembly and disassembly of actin filaments. Defects in this gene are a cause of familial amyloidosis Finnish type (FAF). Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Gelsolin antibody used in Western Blot (WB). GTX114078

GTX114078 WB Image

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Gelsolin antibody (GTX114078) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Gelsolin antibody used in Western Blot (WB). GTX114078

GTX114078 WB Image

Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Gelsolin antibody (GTX114078) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Gelsolin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114078

GTX114078 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using Gelsolin(GTX114078) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-Gelsolin antibody used in IHC (Paraffin sections) (IHC-P). GTX114078

GTX114078 IHC-P Image

Gelsolin antibody detects Gelsolin protein at secreted on mouse urinary bladder by immunohistochemical analysis.
Sample: Paraffin-embedded mouse urinary bladder.
Gelsolin antibody (GTX114078) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Gelsolin antibody used in Western Blot (WB). GTX114078

GTX114078 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Gelsolin antibody (GTX114078) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Gelsolin antibody used in Western Blot (WB). GTX114078

GTX114078 WB Image

Sample (30 μg of whole cell lysate)
A: H1299
B: HeLa
7.5% SDS PAGE
GTX114078 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Gelsolin antibody used in Western Blot (WB). GTX114078

GTX114078 WB Image

Human tissue extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Gelsolin antibody (GTX114078) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Gelsolin antibody used in IHC (Paraffin sections) (IHC-P). GTX114078

GTX114078 IHC-P Image

Gelsolin antibody detects Gelsolin protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Gelsolin antibody (GTX114078) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

There are currently no references for Gelsolin antibody (GTX114078). Be the first to share your publications with this product.

REVIEW

Gelsolin antibody Cat No. GTX114078

Rating ( Average 5 based on 2 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Immunoprecipitation(IP) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 06-Jan-2021
Score :
Application Tested : IP
Sample Species : Hu
Sample : U2OS cells
Figure Note : Immunoprecipitation of Gelsolin protein from U2OS cells using 1 μg of GTX114078 Gelsolin antibody.
SM=10% starting material
UB=10% unbound fraction
Date : Anonymous submitted on 06-Jan-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : U2OS cells
Amount used : 25 μg
Blocking : 5% milk, 1Hr
Primary Antibody : 1:10000, 4°C, 16Hr
SDS
PBS.pdf
Package List Price ($)
$ 329
$ 169