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Gelsolin antibody

Anti-Gelsolin antibody used in Western Blot (WB). GTX114078
Anti-Gelsolin antibody used in Western Blot (WB). GTX114078
Anti-Gelsolin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114078
Anti-Gelsolin antibody used in IHC (Paraffin sections) (IHC-P). GTX114078
Anti-Gelsolin antibody used in Western Blot (WB). GTX114078
Anti-Gelsolin antibody used in Western Blot (WB). GTX114078
Anti-Gelsolin antibody used in Western Blot (WB). GTX114078
Anti-Gelsolin antibody used in IHC (Paraffin sections) (IHC-P). GTX114078

Cat No. GTX114078

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP

Reactivity

Human, Mouse, Rat
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

86 kDa. ( Note )

Positive Control

H1299 , HeLa , human plasma , rat plasma , mouse plasma

Predict Reactivity

Sheep, Bovine, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.13 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Gelsolin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037068

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

gelsolin , ADF , AGEL

Cellular Localization

Isoform 2: Cytoplasm , cytoskeleton , Isoform 1: Secreted

Background

The protein encoded by this gene binds to the "plus" ends of actin monomers and filaments to prevent monomer exchange. The encoded calcium-regulated protein functions in both assembly and disassembly of actin filaments. Defects in this gene are a cause of familial amyloidosis Finnish type (FAF). Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Gelsolin antibody used in Western Blot (WB). GTX114078

GTX114078 WB Image

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Gelsolin antibody (GTX114078) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Gelsolin antibody used in Western Blot (WB). GTX114078

GTX114078 WB Image

Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Gelsolin antibody (GTX114078) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Gelsolin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114078

GTX114078 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using Gelsolin(GTX114078) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-Gelsolin antibody used in IHC (Paraffin sections) (IHC-P). GTX114078

GTX114078 IHC-P Image

Gelsolin antibody detects Gelsolin protein at secreted on mouse urinary bladder by immunohistochemical analysis.
Sample: Paraffin-embedded mouse urinary bladder.
Gelsolin antibody (GTX114078) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Gelsolin antibody used in Western Blot (WB). GTX114078

GTX114078 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Gelsolin antibody (GTX114078) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Gelsolin antibody used in Western Blot (WB). GTX114078

GTX114078 WB Image

Sample (30 μg of whole cell lysate)
A: H1299
B: HeLa
7.5% SDS PAGE
GTX114078 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Gelsolin antibody used in Western Blot (WB). GTX114078

GTX114078 WB Image

Human tissue extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Gelsolin antibody (GTX114078) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Gelsolin antibody used in IHC (Paraffin sections) (IHC-P). GTX114078

GTX114078 IHC-P Image

Gelsolin antibody detects Gelsolin protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Gelsolin antibody (GTX114078) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

REVIEW

Gelsolin antibody Cat No. GTX114078

Rating ( Average 5 based on 2 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Immunoprecipitation(IP) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 06-Jan-2021
Score :
Application Tested : IP
Sample Species : Hu
Sample : U2OS cells
Figure Note : Immunoprecipitation of Gelsolin protein from U2OS cells using 1 μg of GTX114078 Gelsolin antibody.
SM=10% starting material
UB=10% unbound fraction
Date : Anonymous submitted on 06-Jan-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : U2OS cells
Amount used : 25 μg
Blocking : 5% milk, 1Hr
Primary Antibody : 1:10000, 4°C, 16Hr
SDS
PBS.pdf
Package List Price ($)
$ 379
$ 169