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Geminin antibody

Orthogonal
Anti-Geminin antibody used in Immunoprecipitation (IP). GTX116125
Anti-Geminin antibody used in Western Blot (WB). GTX116125
Anti-Geminin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116125
Anti-Geminin antibody used in IHC (Paraffin sections) (IHC-P). GTX116125
Anti-Geminin antibody used in Western Blot (WB). GTX116125

Cat. No. GTX116125

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Geminin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

24 kDa. ( Note )

Positive Control

HCT116

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human Geminin Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10633181

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

geminin DNA replication inhibitor , Gem , MGORS6

Background

Inhibits DNA replication by preventing the incorporation of MCM complex into prereplication complex (pre-RC). It is degraded during the mitotic phase of the cell cycle. Its destruction at the metaphase-anaphase transition permits replication in the succeeding cell cycle.

Database

Research Area

DATA IMAGES

Anti-Geminin antibody used in Immunoprecipitation (IP). GTX116125

GTX116125 IP Image

Immunoprecipitation of Geminin protein from HCT-116 whole cell extracts using 5 μg of Geminin antibody (GTX116125).
Western blot analysis was performed using Geminin antibody (GTX116125) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-Geminin antibody used in Western Blot (WB). GTX116125

GTX116125 WB Image

Sample (30 ug of whole cell lysate)
A: HCT116
12% SDS PAGE
GTX116125 diluted at 1:1000

Anti-Geminin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116125

GTX116125 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Geminin(GTX116125) antibody at 1:500 dilution.

Anti-Geminin antibody used in IHC (Paraffin sections) (IHC-P). GTX116125

GTX116125 IHC-P Image

Geminin antibody detects Geminin protein at nucleus on human breast cancer by immunohistochemical analysis.
Sample: Paraffin-embedded breast cancer.
Geminin antibody (GTX116125) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Geminin antibody used in Western Blot (WB). GTX116125

GTX116125 WB Image

Orthogonal

The data was published in the journal J Virol in 2019.PMID: 31142673

REFERENCE

Application Reference

Caller LG et al. J Virol 2019; 93 (16) Temporal Proteomic Analysis of BK Polyomavirus Infection Reveals Virus-Induced G2 Arrest and Highly Effective Evasion of Innate Immune Sensing.
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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169