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Geminin antibody

Anti-Geminin antibody used in Western Blot (WB). GTX12147

Cat. No. GTX12147

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
200 μl ($399)
See all Geminin products

APPLICATION

Application Note

WB: Use at a dilution of 1/500. Detects a band of approximately 33 kDa (predicted molecular weight: 23.5 kDa). Can be blocked with GTX12408 Geminin peptide . Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

24 kDa. ( Note )

Product Note

This antibody detects a band representing Geminin by Western blot at 33kD on 293T cell lysate, this band can be blocked by the immunising peptide. It also detects a band at about 45kD that is not blocked by the immunising peptide and is thought to be non-specific.

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide: SSENKNLGGVTQES-C conjugated to KLH, corresponding to amino acids 72-85 of Human Geminin.

Purification

Immunogen affinity purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

geminin DNA replication inhibitor , Gem , MGORS6

Cellular Localization

Cytoplasm , Nucleus

Background

This gene encodes a protein that plays a critical role in cell cycle regulation. The encoded protein inhibits DNA replication by binding to DNA replication factor Cdt1, preventing the incorporation of minichromosome maintenance proteins into the pre-replication complex. The encoded protein is expressed during the S and G2 phases of the cell cycle and is degraded by the anaphase-promoting complex during the metaphase-anaphase transition. Increased expression of this gene may play a role in several malignancies including colon, rectal and breast cancer. Alternatively spliced transcript variants have been observed for this gene, and two pseudogenes of this gene are located on the short arm of chromosome 16. [provided by RefSeq, Oct 2011]

Database

Research Area

DATA IMAGES

Anti-Geminin antibody used in Western Blot (WB). GTX12147

GTX12147 WB Image

WB analysis of 293 lysate using Geminin antibody in the abscence (lane 1) or prescence (lane 2) of blocking peptide.

REFERENCE

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399