GeneTex
United States (US)

Geminin antibody

Anti-Geminin antibody used in Western Blot (WB). GTX33218
Anti-Geminin antibody used in IHC (Paraffin sections) (IHC-P). GTX33218

Cat No. GTX33218

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

24 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-209 of human GMNN (NP_056979.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Geminin, Dna Replication Inhibitor,Gem,Mgors6,Gmnn

Background

This gene encodes a protein that plays a critical role in cell cycle regulation. The encoded protein inhibits DNA replication by binding to DNA replication factor Cdt1, preventing the incorporation of minichromosome maintenance proteins into the pre-replication complex. The encoded protein is expressed during the S and G2 phases of the cell cycle and is degraded by the anaphase-promoting complex during the metaphase-anaphase transition. Increased expression of this gene may play a role in several malignancies including colon, rectal and breast cancer. Alternatively spliced transcript variants have been observed for this gene, and two pseudogenes of this gene are located on the short arm of chromosome 16. [provided by RefSeq, Oct 2011]

Database

Research Area

DATA IMAGES
Anti-Geminin antibody used in Western Blot (WB). GTX33218

GTX33218 WB Image

WB analysis of various samples using GTX33218 Geminin antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-Geminin antibody used in IHC (Paraffin sections) (IHC-P). GTX33218

GTX33218 IHC-P Image

IHC-P analysis of human liver cancer tissue using GTX33218 Geminin antibody.
Dilution : 1:100