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Gephyrin antibody [N2C1], Internal

Anti-Gephyrin antibody [N2C1], Internal used in IHC (Frozen sections) (IHC-Fr). GTX109734
Anti-Gephyrin antibody [N2C1], Internal used in Immunoprecipitation (IP). GTX109734
Anti-Gephyrin antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX109734
Anti-Gephyrin antibody [N2C1], Internal used in Western Blot (WB). GTX109734
Anti-Gephyrin antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109734
Anti-Gephyrin antibody [N2C1], Internal used in Western Blot (WB). GTX109734
Anti-Gephyrin antibody [N2C1], Internal used in Western Blot (WB). GTX109734
Anti-Gephyrin antibody [N2C1], Internal used in Western Blot (WB). GTX109734

Cat No. GTX109734

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr, IP

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

80 kDa. ( Note )

Positive Control

NIH-3T3 , JC , U87-MG , SK-N-SH , IMR-32 , SK-N-AS , mouse brain , rat brain

Predict Reactivity

Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.48 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Gephyrin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11164321

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

gephyrin , GEPH , GPH , GPHRYN , HKPX1 , MOCODC

Cellular Localization

Cell junction , synapse , synapse , postsynaptic cell membrane; Peripheral membrane protein; Cytoplasmic side , Cytoplasm , cytoskeleton

Background

This gene encodes a neuronal assembly protein that anchors inhibitory neurotransmitter receptors to the postsynaptic cytoskeleton via high affinity binding to a receptor subunit domain and tubulin dimers. In nonneuronal tissues, the encoded protein is also required for molybdenum cofactor biosynthesis. Mutations in this gene may be associated with the neurological condition hyperplexia and also lead to molybdenum cofactor deficiency. Numerous alternatively spliced transcript variants encoding different isoforms have been described; however, the full-length nature of all transcript variants is not currently known. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Gephyrin antibody [N2C1], Internal used in IHC (Frozen sections) (IHC-Fr). GTX109734

GTX109734 IHC-Fr Image

Gephyrin antibody [N2C1], Internal detects Gephyrin protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: Gephyrin protein stained by Gephyrin antibody [N2C1], Internal (GTX109734) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Gephyrin antibody [N2C1], Internal used in Immunoprecipitation (IP). GTX109734

GTX109734 IP Image

Immunoprecipitation of Gephyrin protein from A431 whole cell extracts using 5 μg of Gephyrin antibody [N2C1], Internal (GTX109734).
Western blot analysis was performed using Gephyrin antibody [N2C1], Internal (GTX109734).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-Gephyrin antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX109734

GTX109734 IHC-P Image

Immunohistochemical analysis of paraffin-embedded mouse brain, using Gephyrin(GTX109734) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Gephyrin antibody [N2C1], Internal used in Western Blot (WB). GTX109734

GTX109734 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Gephyrin antibody [N2C1], Internal (GTX109734) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Gephyrin antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109734

GTX109734 ICC/IF Image

Gephyrin antibody [N2C1], Internal detects Gephyrin protein by immunofluorescent analysis.
Sample: DIV10 rat E18 primary hippocampal neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Gephyrin protein stained by Gephyrin antibody [N2C1], Internal (GTX109734) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Gephyrin antibody [N2C1], Internal used in Western Blot (WB). GTX109734

GTX109734 WB Image

Sample (30 ug of whole cell lysate)
A: NIH-3T3
B: JC
7.5% SDS PAGE
GTX109734 diluted at 1:1000

Anti-Gephyrin antibody [N2C1], Internal used in Western Blot (WB). GTX109734

GTX109734 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Gephyrin antibody [N2C1], Internal (GTX109734) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Gephyrin antibody [N2C1], Internal used in Western Blot (WB). GTX109734

GTX109734 WB Image

Human tissue extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Gephyrin antibody [N2C1], Internal (GTX109734) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169