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Germ cell-specific antigen antibody [TRA98]

Orthogonal
Anti-Germ cell-specific antigen antibody [TRA98] used in Western Blot (WB). GTX00717
Anti-Germ cell-specific antigen antibody [TRA98] used in IHC (Frozen sections) (IHC-Fr). GTX00717
Anti-Germ cell-specific antigen antibody [TRA98] used in IHC (Paraffin sections) (IHC-P). GTX00717

Cat. No. GTX00717

Reference 3

Host

 Rat

Clonality

 Monoclonal

Clone Name

 TRA98

Isotype

 IgG2a

Application

 WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, IHC

Reactivity

 Mouse
Package
100 μg ($399)
See all Germ cell-specific antigen products
PRODUCT

Summary

This antibody recognizes a mouse testicular germ cell-specific antigen. In adult tissues, bands of 60-100 kDa proteins are detected only in the testis by Western blotting analysis with this antibody. The signals are observed in male and female embryos after embryonic day 12.5. The signal in male is detected during development of germ cells and also after birth, but the signal in female disappears by 5 days after birth. The antigen is localized only in the nuclear fraction of testicular germ cells and this antibody can be used for immunohistochemical staining of testicular germ cells.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:5000
ICC/IF 1:400
IHC-P 1:200-1:500
IHC-Fr 1:200-1:500
FACS Assay dependent
IP 1:200
IHC Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

adult mouse testis

Purification

Purified IgG

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-Germ cell-specific antigen antibody [TRA98] used in Western Blot (WB). GTX00717

GTX00717 WB Image

Orthogonal

WB analysis of various mouse tissue samples using GTX00717 Germ cell-specific antigen antibody [TRA98].
This protien is testis-specific.
Dilution : 1 μg/ml
Loading : 100 μg
This protien is testis-specific.

Anti-Germ cell-specific antigen antibody [TRA98] used in IHC (Frozen sections) (IHC-Fr). GTX00717

GTX00717 IHC-Fr Image

IHC-Fr analysis of mouse testis tissue section (7-day-old) using GTX00717 Germ cell-specific antigen antibody [TRA98].
Scale bar : 50 μm

Anti-Germ cell-specific antigen antibody [TRA98] used in IHC (Paraffin sections) (IHC-P). GTX00717

GTX00717 IHC-P Image

IHC-P analysis of mouse testis tissue section (10-day-old) using GTX00717 Germ cell-specific antigen antibody [TRA98]. Anti-GATA antibody stains nuclei of Sertoli cells in seminiferous tubule while anti-TRA54 antibody stains reproductive cells.
Dilution : 1:400
Antigen retrieval : 10 mM citrate buffer (pH 6.0) for 15min
Fixation : 4% PFA

REFERENCE

Clone Reference

Fukunaga H et al. Radiat Res. 2018; 189 (6):661-667 Application of an Ex Vivo Tissue Model to Investigate Radiobiological Effects on Spermatogenesis.
Application : IHC
Reactivity : Mouse
Gu W et al. Biol Open. 2018; 7 (1) Dnd1-mediated epigenetic control of teratoma formation in mouse.
Application : IHC-Fr
Reactivity : Mouse
Krentz AD et al. Dev Biol. 2011; 356 (1):63-70 DMRT1 promotes oogenesis by transcriptional activation of Stra8 in the mammalian fetal ovary.
Application : ICC/IF, IHC-P
Reactivity : Mouse
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Package List Price ($)
$ 399