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Gfap antibody

Anti-Gfap antibody used in Western Blot (WB). GTX128644
Anti-Gfap antibody used in IHC (Whole mount) (IHC-Wm). GTX128644

Cat No. GTX128644

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-Wm

Reactivity

Zebrafish
Package
200 μl ($409),
50 μl ($219)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-Wm 1:100-1:500
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

Positive Control

24hpf zebrafish embryos

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.87 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Zebrafish

Immunogen

Recombinant protein encompassing a sequence within the center region of zebrafish GFAP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

glial fibrillary acidic protein , cb345 , etID36982.3 , gfapl , wu:fb34h11 , wu:fk42c12 , xx:af506734 , zgc:110485

Cellular Localization

Cytoplasm , cytoskeleton

Database

Research Area

DATA IMAGES

Anti-Gfap antibody used in Western Blot (WB). GTX128644

GTX128644 WB Image

Gfap antibody detects Gfap protein by western blot analysis. Zebrafish tissue extracts (30 μg) was separated by 10 % SDS-PAGE, and the membrane was blotted with Gfap antibody (GTX128644) at a dilution of 1:1000.

Anti-Gfap antibody used in IHC (Whole mount) (IHC-Wm). GTX128644

GTX128644 IHC-Wm Image

Gfap antibody detects Gfap protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 1 day-post-fertilization zebrafish embryo.
Gfap antibody (GTX128644) dilution: 1:100.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 409
$ 219