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Gfap antibody

Anti-Gfap antibody used in IHC (Whole mount) (IHC-Wm). GTX128741
Anti-Gfap antibody used in IHC (Whole mount) (IHC-Wm). GTX128741
Anti-Gfap antibody used in Western Blot (WB). GTX128741
Anti-Gfap antibody used in IHC (Frozen sections) (IHC-Fr). GTX128741
Anti-Gfap antibody used in IHC (Frozen sections) (IHC-Fr). GTX128741
Anti-Gfap antibody used in IHC (Frozen sections) (IHC-Fr). GTX128741
Anti-Gfap antibody used in IHC (Frozen sections) (IHC-Fr). GTX128741

Cat. No. GTX128741

Reference 13

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-Fr, IHC-Wm

Reactivity

 Zebrafish, Fish
Package
100 μl ($399),
25 μl ($169)
See all zebrafish Gfap products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-Fr Assay dependent
IHC-Wm 1:100-1:500
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

Positive Control

zebrafish embryos

Predict Reactivity

Mouse(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.32 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Zebrafish

Immunogen

Recombinant protein encompassing a sequence within the center region of zebrafish gfap. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2814877

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glial fibrillary acidic protein , cb345 , etID36982.3 , gfapl , wu:fb34h11 , wu:fk42c12 , xx:af506734 , zgc:110485

Cellular Localization

Cytoplasm , cytoskeleton

Database

Research Area

DATA IMAGES

Anti-Gfap antibody used in IHC (Whole mount) (IHC-Wm). GTX128741

GTX128741 IHC-Wm Image

Gfap antibody detects Gfap protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 1 day-post-fertilization zebrafish embryo.
Gfap antibody (GTX128741) dilution: 1:100.
Anti-Gfap antibody used in IHC (Whole mount) (IHC-Wm). GTX128741

GTX128741 IHC-Wm Image

Gfap antibody detects Gfap protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 1 day-post-fertilization zebrafish embryo.
Gfap antibody (GTX128741) dilution: 1:100.
Anti-Gfap antibody used in Western Blot (WB). GTX128741

GTX128741 WB Image

Zebrafish embryo extracts (10 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Gfap antibody (GTX128741) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Gfap antibody used in IHC (Frozen sections) (IHC-Fr). GTX128741

GTX128741 IHC-Fr Image

The data was published in the journal Sci Rep in 2019.PMID: 31061452

Anti-Gfap antibody used in IHC (Frozen sections) (IHC-Fr). GTX128741

GTX128741 IHC-Fr Image

The data was published in the journal Sci Rep in 2019.PMID: 31061452

Anti-Gfap antibody used in IHC (Frozen sections) (IHC-Fr). GTX128741

GTX128741 IHC-Fr Image

The data was published in the journal Sci Rep in 2019.PMID: 31061452

Anti-Gfap antibody used in IHC (Frozen sections) (IHC-Fr). GTX128741

GTX128741 IHC-Fr Image

The data was published in the journal Sci Rep in 2019.PMID: 31061452

REFERENCE

Application Reference

NA Omar et al. Front Pharmacol 2023; 14 : 1307447 Neuroprotective effects of Neurotrophin-3 in MPTP-induced zebrafish Parkinson’s disease model
Sun K et al. iScience 2023; 26 (11) : 108103 The splicing factor DHX38 enables retinal development through safeguarding genome integrity.
Titialii-Torres KF et al. J Cell Sci 2022; 135 (1) Embryonic hyperglycemia perturbs the development of specific retinal cell types, including photoreceptors.
Valerio Laghi et al. Front Cell Infect Microbiol 2022; 12 : 790851 Exploring Zebrafish Larvae as a COVID-19 Model: Probable Abortive SARS-CoV-2 Replication in the Swim Bladder.
Application : IHC-Wm
Kehua Chen et al. Biomedicines 2022; 10 (2) Loss of atm in Zebrafish as a Model of Ataxia-Telangiectasia Syndrome.
Application : IHC-Fr
Reactivity : Zebrafish
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REVIEW

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Application Reference
NA Omar et al. Front Pharmacol 2023; 14:1307447 Neuroprotective effects of Neurotrophin-3 in MPTP-induced zebrafish Parkinson’s disease model
Sun K et al. iScience 2023; 26 (11):108103 The splicing factor DHX38 enables retinal development through safeguarding genome integrity.
Titialii-Torres KF et al. J Cell Sci 2022; 135 (1) Embryonic hyperglycemia perturbs the development of specific retinal cell types, including photoreceptors.
Valerio Laghi et al. Front Cell Infect Microbiol 2022; 12:790851 Exploring Zebrafish Larvae as a COVID-19 Model: Probable Abortive SARS-CoV-2 Replication in the Swim Bladder.
Application : IHC-Wm
Kehua Chen et al. Biomedicines 2022; 10 (2) Loss of atm in Zebrafish as a Model of Ataxia-Telangiectasia Syndrome.
Application : IHC-Fr
Reactivity : Zebrafish
Pereida-Jaramillo E et al. Int J Mol Sci 2021; 22 (24) Calcium Signaling in the Cerebellar Radial Glia and Its Association with Morphological Changes during Zebrafish Development.
Ahmad MH et al. Neuropharmacology 2021; 201:108831 Naringenin alleviates paraquat-induced dopaminergic neuronal loss in SH-SY5Y cells and a rat model of Parkinson's disease.
McCallum S et al. Elife 2020; 9 Enteric glia as a source of neural progenitors in adult zebrafish.
Emam A et al. J Comp Neurol 2020; 528 (9):1523-1534 Retinal morphology in Astyanax mexicanus during eye degeneration.
Ruiz-Palacios M et al. Fish Shellfish Immunol 2020; 106:161-166 Establishment of a brain cell line (SaB-1) from gilthead seabream and its application to fish virology.
Application : ICC/IF
Reactivity : Fish
Zhang J et al. Nat Commun 2020; 11 (1):1860 Wnt-PLC-IP 3-Connexin-Ca 2+ axis maintains ependymal motile cilia in zebrafish spinal cord.
Baker PA et al. Sci Rep 2019; 9 (1):6941 Immunohistochemical and ultrastructural analysis of the maturing larval zebrafish enteric nervous system reveals the formation of a neuropil pattern.
Phillip A Baker et al. bioRxiv 2019; (Epub) Immunohistochemical and ultrastructural analysis of the maturing larval zebrafish enteric nervous system reveals the formation of a neuropil pattern.
Application : IHC-Fr
Reactivity : Zebrafish
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169