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Gfap antibody

Anti-Gfap antibody used in IHC (Whole mount) (IHC-Wm). GTX128741
Anti-Gfap antibody used in IHC (Whole mount) (IHC-Wm). GTX128741
Anti-Gfap antibody used in Western Blot (WB). GTX128741

Cat No. GTX128741

Reference 13
4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-Fr, IHC-Wm

Reactivity

 Zebrafish, Fish
Package
100 μl ($409),
25 μl ($219)
See all zebrafish Gfap products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-Fr Assay dependent
IHC-Wm 1:100-1:500
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

Positive Control

zebrafish embryos

Predict Reactivity

Mouse(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.32 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Zebrafish

Immunogen

Recombinant protein encompassing a sequence within the center region of zebrafish gfap. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2814877

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

glial fibrillary acidic protein , cb345 , etID36982.3 , gfapl , wu:fb34h11 , wu:fk42c12 , xx:af506734 , zgc:110485

Cellular Localization

Cytoplasm , cytoskeleton

Database

Research Area

DATA IMAGES

Anti-Gfap antibody used in IHC (Whole mount) (IHC-Wm). GTX128741

GTX128741 IHC-Wm Image

Gfap antibody detects Gfap protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 1 day-post-fertilization zebrafish embryo.
Gfap antibody (GTX128741) dilution: 1:100.
Anti-Gfap antibody used in IHC (Whole mount) (IHC-Wm). GTX128741

GTX128741 IHC-Wm Image

Gfap antibody detects Gfap protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 1 day-post-fertilization zebrafish embryo.
Gfap antibody (GTX128741) dilution: 1:100.
Anti-Gfap antibody used in Western Blot (WB). GTX128741

GTX128741 WB Image

gfap antibody detects gfap protein by western blot analysis. Zebrafish tissue extracts (30 μg) was separated by 10 % SDS-PAGE, and the membrane was blotted with gfap antibody (GTX128741) at a dilution of 1:1000.

REFERENCE

Application Reference

Valerio Laghi et al. Front Cell Infect Microbiol 2022; 12 : 790851 Exploring Zebrafish Larvae as a COVID-19 Model: Probable Abortive SARS-CoV-2 Replication in the Swim Bladder.
Kehua Chen et al. Biomedicines 2022; 10 (2) Loss of atm in Zebrafish as a Model of Ataxia-Telangiectasia Syndrome.
Pereida-Jaramillo E et al. Int J Mol Sci 2021; 22 (24) Calcium Signaling in the Cerebellar Radial Glia and Its Association with Morphological Changes during Zebrafish Development.
Ahmad MH et al. Neuropharmacology 2021; 201 : 108831 Naringenin alleviates paraquat-induced dopaminergic neuronal loss in SH-SY5Y cells and a rat model of Parkinson's disease.
McCallum S et al. Elife 2020; 9 Enteric glia as a source of neural progenitors in adult zebrafish.
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Submit a Reference

REVIEW

Gfap antibody Cat No. GTX128741

Rating
( Average 5 based on 4 users reviews)
Application
IHC (Frozen sections)(IHC-Fr)
( Average 5 based on 4 users reviews)
Anti-Gfap antibody used in IHC (Frozen sections) (IHC-Fr). GTX128741
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : IHC-Fr
Sample Species : Zfsh
Figure Note : The data was peer reviewed and published in the journal Elife in 2018. PMID: 29809141
Anti-Gfap antibody used in IHC (Frozen sections) (IHC-Fr). GTX128741
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : IHC-Fr
Sample Species : Zfsh
Figure Note : The data was peer reviewed and published in the journal Elife in 2018. PMID: 29809141
Anti-Gfap antibody used in IHC (Frozen sections) (IHC-Fr). GTX128741
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : IHC-Fr
Sample Species : Zfsh
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2019. PMID: 31757956
Anti-Gfap antibody used in IHC (Frozen sections) (IHC-Fr). GTX128741
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : IHC-Fr
Sample Species : Zfsh
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2018. PMID: 29472538
Submit a Review
Application Reference
Valerio Laghi et al. Front Cell Infect Microbiol 2022; 12:790851 Exploring Zebrafish Larvae as a COVID-19 Model: Probable Abortive SARS-CoV-2 Replication in the Swim Bladder.
Kehua Chen et al. Biomedicines 2022; 10 (2) Loss of atm in Zebrafish as a Model of Ataxia-Telangiectasia Syndrome.
Pereida-Jaramillo E et al. Int J Mol Sci 2021; 22 (24) Calcium Signaling in the Cerebellar Radial Glia and Its Association with Morphological Changes during Zebrafish Development.
Ahmad MH et al. Neuropharmacology 2021; 201:108831 Naringenin alleviates paraquat-induced dopaminergic neuronal loss in SH-SY5Y cells and a rat model of Parkinson's disease.
McCallum S et al. Elife 2020; 9 Enteric glia as a source of neural progenitors in adult zebrafish.
Emam A et al. J Comp Neurol 2020; 528 (9):1523-1534 Retinal morphology in Astyanax mexicanus during eye degeneration.
Ruiz-Palacios M et al. Fish Shellfish Immunol 2020; 106:161-166 Establishment of a brain cell line (SaB-1) from gilthead seabream and its application to fish virology.
Application : ICC/IF
Reactivity : Fish
Zhang J et al. Nat Commun 2020; 11 (1):1860 Wnt-PLC-IP 3-Connexin-Ca 2+ axis maintains ependymal motile cilia in zebrafish spinal cord.
Wootae Kim et al. Nat Commun 2019; 10 (1):5304 ZFP161 regulates replication fork stability and maintenance of genomic stability by recruiting the ATR/ATRIP complex.
Application : IHC-Fr
Reactivity : Zebrafish
Baker PA et al. Sci Rep 2019; 9 (1):6941 Immunohistochemical and ultrastructural analysis of the maturing larval zebrafish enteric nervous system reveals the formation of a neuropil pattern.
Phillip A Baker et al. bioRxiv 2019; (Epub) Immunohistochemical and ultrastructural analysis of the maturing larval zebrafish enteric nervous system reveals the formation of a neuropil pattern.
Application : IHC-Fr
Reactivity : Zebrafish
Anja G?der et al. Nat Commun 2018; 9 (1):764 HDAC1 and HDAC2 integrate checkpoint kinase phosphorylation and cell fate through the phosphatase-2A subunit PR130.
Application : IHC-Fr
Reactivity : Zebrafish
Hajime Fukui et al. Elife 2018; 7 (0) Hippo signaling determines the number of venous pole cells that originate from the anterior lateral plate mesoderm in zebrafish.
Application : IHC-Fr
Reactivity : Zebrafish
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 409
$ 219