Gfap antibody

Cat No. GTX128741

Reference 4

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-Fr, IHC-Wm
Reactivity	Zebrafish, Fish
Package	200 μl ($409), 50 μl ($219)

See all zebrafish Gfap products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:500-1:3000
ICC/IF	Assay dependent
IHC-Fr	Assay dependent
IHC-Wm	1:100-1:500

Not tested in other applications.

Calculated MW

51 kDa. ( Note )

Positive Control

zebrafish embryos

Predict Reactivity

Mouse(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.34 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Zebrafish

Immunogen

Recombinant protein encompassing a sequence within the center region of zebrafish gfap. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2814877

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

glial fibrillary acidic protein , cb345 , etID36982.3 , gfapl , wu:fb34h11 , wu:fk42c12 , xx:af506734 , zgc:110485

Cellular Localization

Cytoplasm , cytoskeleton

Database

Gene ID: 30646 gfap
UniProt: Q58EE9 gfap

Research Area

- Zebrafish

DATA IMAGES

GTX128741 IHC-Wm Image

Gfap antibody detects Gfap protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 1 day-post-fertilization zebrafish embryo.
Gfap antibody (GTX128741) dilution: 1:100.

GTX128741 IHC-Wm Image

Gfap antibody detects Gfap protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 1 day-post-fertilization zebrafish embryo.
Gfap antibody (GTX128741) dilution: 1:100.

GTX128741 WB Image

gfap antibody detects gfap protein by western blot analysis. Zebrafish tissue extracts (30 μg) was separated by 10 % SDS-PAGE, and the membrane was blotted with gfap antibody (GTX128741) at a dilution of 1:1000.