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Gfap antibody

Anti-Gfap antibody used in IHC (Whole mount) (IHC-Wm). GTX128741
Anti-Gfap antibody used in IHC (Whole mount) (IHC-Wm). GTX128741
Anti-Gfap antibody used in Western Blot (WB). GTX128741

Cat No. GTX128741

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-Fr, IHC-Wm

Reactivity

 Zebrafish
Package
200 μl ($409),
50 μl ($219)
See all zebrafish Gfap products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-Fr Assay dependent
IHC-Wm 1:100-1:500
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

Positive Control

zebrafish embryos

Predict Reactivity

Mouse(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.34 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Zebrafish

Immunogen

Recombinant protein encompassing a sequence within the center region of zebrafish gfap. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

glial fibrillary acidic protein , cb345 , etID36982.3 , gfapl , wu:fb34h11 , wu:fk42c12 , xx:af506734 , zgc:110485

Cellular Localization

Cytoplasm , cytoskeleton

Database

Research Area

DATA IMAGES

Anti-Gfap antibody used in IHC (Whole mount) (IHC-Wm). GTX128741

GTX128741 IHC-Wm Image

Gfap antibody detects Gfap protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 1 day-post-fertilization zebrafish embryo.
Gfap antibody (GTX128741) dilution: 1:100.
Anti-Gfap antibody used in IHC (Whole mount) (IHC-Wm). GTX128741

GTX128741 IHC-Wm Image

Gfap antibody detects Gfap protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 1 day-post-fertilization zebrafish embryo.
Gfap antibody (GTX128741) dilution: 1:100.
Anti-Gfap antibody used in Western Blot (WB). GTX128741

GTX128741 WB Image

gfap antibody detects gfap protein by western blot analysis. Zebrafish tissue extracts (30 μg) was separated by 10 % SDS-PAGE, and the membrane was blotted with gfap antibody (GTX128741) at a dilution of 1:1000.

REFERENCE

Application Reference

Zhang J et al. Nat Commun 2020; 11 (1) : 1860 Wnt-PLC-IP 3-Connexin-Ca 2+ axis maintains ependymal motile cilia in zebrafish spinal cord.
Baker PA et al. Sci Rep 2019; 9 (1) : 6941 Immunohistochemical and ultrastructural analysis of the maturing larval zebrafish enteric nervous system reveals the formation of a neuropil pattern.
Phillip A Baker et al. bioRxiv 2019; (Epub) Immunohistochemical and ultrastructural analysis of the maturing larval zebrafish enteric nervous system reveals the formation of a neuropil pattern.
Application : IHC-Fr
Reactivity : Zebrafish
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 409
$ 219