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Ghrelin antibody

Anti-Ghrelin antibody used in Western Blot (WB). GTX33964
Anti-Ghrelin antibody used in Western Blot (WB). GTX33964
Anti-Ghrelin antibody used in IHC (Paraffin sections) (IHC-P). GTX33964

Cat. No. GTX33964

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399)
See all Ghrelin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
IHC-P 1:100-1:300
Not tested in other applications.

Calculated MW

13 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.5% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthesized peptide derived from human Ghrelin.

Purification

Purified by antigen-affinity chromatography
From serum

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

GHRL,MTLRP,ghrelin and obestatin prepropeptide,Ghrelin,ghrelin/obestatin prepropeptide

Cellular Localization

Secreted

Background

This gene encodes the ghrelin-obestatin preproprotein that is cleaved to yield two peptides, ghrelin and obestatin. Ghrelin is a powerful appetite stimulant and plays an important role in energy homeostasis. Its secretion is initiated when the stomach is empty, whereupon it binds to the growth hormone secretagogue receptor in the hypothalamus which results in the secretion of growth hormone (somatotropin). Ghrelin is thought to regulate multiple activities, including hunger, reward perception via the mesolimbic pathway, gastric acid secretion, gastrointestinal motility, and pancreatic glucose-stimulated insulin secretion. It was initially proposed that obestatin plays an opposing role to ghrelin by promoting satiety and thus decreasing food intake, but this action is still debated. Recent reports suggest multiple metabolic roles for obestatin, including regulating adipocyte function and glucose metabolism. Alternative splicing results in multiple transcript variants. In addition, antisense transcripts for this gene have been identified and may potentially regulate ghrelin-obestatin preproprotein expression. [provided by RefSeq, Nov 2014]

Database

Research Area

DATA IMAGES

Anti-Ghrelin antibody used in Western Blot (WB). GTX33964

GTX33964 WB Image

WB analysis of 293 cell lysate using GTX33964 Ghrelin antibody.

Anti-Ghrelin antibody used in Western Blot (WB). GTX33964

GTX33964 WB Image

WB analysis of 293 cell lysate using GTX33964 Ghrelin antibody.

Anti-Ghrelin antibody used in IHC (Paraffin sections) (IHC-P). GTX33964

GTX33964 IHC-P Image

IHC-P analysis of human lung cancer tissue using GTX33964 Ghrelin antibody. Negative control (the lower left coner) was secondary antibody only.
Antigen retrieval : Tris-EDTA, pH8.0 under high-pressure and temperature
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399