APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:50-1:100 |
Not tested in other applications.
Calculated MW
Predict Reactivity
Mouse(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide sequence around phosphorylation site of Serine 1417(Q-K-S(p)-L-T) derived from human Girdin.
Purification
Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
coiled-coil domain containing 88A , APE , GIRDIN , GIV , GRDN , HkRP1 , KIAA1212 , PEHO , PEHOL
Cellular Localization
Membrane,Cell membrane,Cytoplasm, cytosol,Cytoplasmic vesicle
Background
This gene encodes a member of the Girdin family of coiled-coil domain containing proteins. The encoded protein is an actin-binding protein that is activated by the serine/threonine kinase Akt and plays a role in cytoskeleton remodeling and cell migration. The encoded protein also enhances Akt signaling by mediating phosphoinositide 3-kinase (PI3K)-dependent activation of Akt by growth factor receptor tyrosine kinases and G protein-coupled receptors. Increased expression of this gene and phosphorylation of the encoded protein may play a role in cancer metastasis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011]
Database
Research Area
DATA IMAGES
|
GTX55363 IHC-P Image
IHC-P analysis of human breast carcinoma tissue using GTX55363 Girdin (phospho Ser1417) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide
|
REFERENCE
There are currently no references for Girdin (phospho Ser1417) antibody (GTX55363). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Girdin (phospho Ser1417) antibody (GTX55363). Be the first to share your experience with this product.
