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Gli1 antibody [HL247]

Recombinant antibody KO/KD Overexpression
Anti-Gli1 antibody [HL247] used in Western Blot (WB). GTX635619
Anti-Gli1 antibody [HL247] used in IHC (Paraffin sections) (IHC-P). GTX635619
Anti-Gli1 antibody [HL247] used in Western Blot (WB). GTX635619
Anti-Gli1 antibody [HL247] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635619
Anti-Gli1 antibody [HL247] used in IHC (Paraffin sections) (IHC-P). GTX635619
Anti-Gli1 antibody [HL247] used in Western Blot (WB). GTX635619

Cat. No. GTX635619

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL247

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Gli1 products
PRODUCT

Summary

Gli1 antibody detects Gli1 protein, a zinc-finger protein with a predicted molecular weight of 119 kDa. Gli1 is a transcription factor that serves as an effector of Sonic hedgehog signaling in mediating cell fate during embryonic development. In addition, Gli1 has been shown to control many basic cell functions including the cell cycle, growth, and cell death. Although Gli1 was originally isolated from a glioma tumor, its upregulation is found in many other tumor types.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

119 kDa. ( Note )

Predict Reactivity

Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of mouse Gli1. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888543

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

GLI-Kruppel family member GLI1 , AV235269 , Zfp-5 , Zfp5

Cellular Localization

Cytoplasm,Nucleus

Database

Research Area

DATA IMAGES

Anti-Gli1 antibody [HL247] used in Western Blot (WB). GTX635619

GTX635619 WB Image

KO/KD

Non-transfected (–) and transfected (+) RMS13 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Gli1 antibody [HL247] (GTX635619) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Gli1 antibody [HL247] used in IHC (Paraffin sections) (IHC-P). GTX635619

GTX635619 IHC-P Image

Gli1 antibody [HL247] detects Gli1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cerebellum.
Gli1 stained by Gli1 antibody [HL247] (GTX635619) diluted at 1:50.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Gli1 antibody [HL247] used in Western Blot (WB). GTX635619

GTX635619 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Gli1 antibody [HL247] (GTX635619) diluted at 1:1000000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Gli1 antibody [HL247] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635619

GTX635619 ICC/IF Image

Overexpression

Gli1 antibody [HL247] detects Gli1 protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Gli1 stained by Gli1 antibody [HL247] (GTX635619) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Gli1 antibody [HL247] used in IHC (Paraffin sections) (IHC-P). GTX635619

GTX635619 IHC-P Image

Gli1 antibody [HL27] detects Gli1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissues.
Gli1 stained by Gli1 antibody [HL27] (GTX635619) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Gli1 antibody [HL247] used in Western Blot (WB). GTX635619

GTX635619 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Gli1 antibody [HL27] (GTX635619) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations