Gli1 antibody [HL247]

Name: Gli1 antibody [HL247]
Brand: GeneTex
SKU: GTX635619
Price: 399.00 USD

Anti-Gli1 antibody [HL247] used in Western Blot (WB). GTX635619

Anti-Gli1 antibody [HL247] used in IHC (Paraffin sections) (IHC-P). GTX635619

Anti-Gli1 antibody [HL247] used in Immunoprecipitation (IP). GTX635619

Anti-Gli1 antibody [HL247] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635619

Cat. No. GTX635619

Host	Rabbit
Clonality	Monoclonal
Clone Name	HL247
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IP
Reactivity	Human, Mouse, Rat
Package	100 μl ($399), 25 μl ($169)

See all Gli1 products

Datasheet

View product citations for antibody GTX635619 on CiteAb

PRODUCT

Summary

Gli1 antibody detects Gli1 protein, a zinc-finger protein with a predicted molecular weight of 119 kDa. Gli1 is a transcription factor that serves as an effector of Sonic hedgehog signaling in mediating cell fate during embryonic development. In addition, Gli1 has been shown to control many basic cell functions including the cell cycle, growth, and cell death. Although Gli1 was originally isolated from a glioma tumor, its upregulation is found in many other tumor types.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	Assay dependent
IHC-P	Assay dependent
IP	Assay dependent

Not tested in other applications.

Calculated MW

119 kDa. ( Note )

Predict Reactivity

Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of mouse Gli1. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888543

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

GLI-Kruppel family member GLI1 , AV235269 , Zfp-5 , Zfp5

Cellular Localization

Cytoplasm,Nucleus

Database

Gene ID: 14632 Gli1
UniProt: P47806 Gli1

Research Area

DATA IMAGES

GTX635619 WB Image

Non-transfected (–) and transfected (+) RMS13 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Gli1 antibody [HL247] (GTX635619) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX635619 IHC-P Image

Gli1 antibody [HL247] detects Gli1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cerebellum.
Gli1 stained by Gli1 antibody [HL247] (GTX635619) diluted at 1:50.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX635619 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Gli1 antibody [HL247] (GTX635619) diluted at 1:1000000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX635619 WB Image

RMS 13 whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Gli1 antibody [HL247] (GTX635619) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX635619 IHC-P Image

Gli1 antibody [HL27] detects Gli1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissues.
Gli1 stained by Gli1 antibody [HL27] (GTX635619) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX635619 IP Image

Immunoprecipitation of Gli1 protein from RMS 13 whole cell extract using 5 μg of Gli1 antibody [HL247] (GTX635619).
Western blot analysis was performed using Gli1 antibody [HL247] (GTX635619).
EasyBlot HRP-conjugated anti rabbit IgG antibody (GTX221666-01).

GTX635619 ICC/IF Image

Gli1 antibody [HL247] detects Gli1 protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Gli1 stained by Gli1 antibody [HL247] (GTX635619) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX635619 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Gli1 antibody [HL27] (GTX635619) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty Custom Conjugations

Gli1 antibody [HL247]

Cat. No. GTX635619

Host

Clonality

Clone Name

Isotype

Application

Reactivity

Summary

APPLICATION

Application Note

Calculated MW

Predict Reactivity

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

RRID

Note

TARGET

Synonyms

Cellular Localization

Database

Research Area

DATA IMAGES

GTX635619 WB Image

GTX635619 IHC-P Image

GTX635619 WB Image

GTX635619 WB Image

GTX635619 IHC-P Image

GTX635619 IP Image

GTX635619 ICC/IF Image

GTX635619 WB Image

REFERENCE

REVIEW

Product

Research

Support

Company

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All products are for research use only—Not for use in diagnostic or therapeutic applications.