PRODUCT
Summary
Gli1 antibody detects Gli1 protein, a zinc-finger protein with a predicted molecular weight of 119 kDa. Gli1 is a transcription factor that serves as an effector of Sonic hedgehog signaling in mediating cell fate during embryonic development. In addition, Gli1 has been shown to control many basic cell functions including the cell cycle, growth, and cell death. Although Gli1 was originally isolated from a glioma tumor, its upregulation is found in many other tumor types.
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| Assay dependent |
Not tested in other applications.
Calculated MW
Predict Reactivity
Bovine, Pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Mouse
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of mouse Gli1. The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
RRID
AB_2888543
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
GLI-Kruppel family member GLI1 , AV235269 , Zfp-5 , Zfp5
Cellular Localization
Cytoplasm,Nucleus
Database
Research Area
DATA IMAGES
![Anti-Gli1 antibody [HL247] used in Western Blot (WB). GTX635619](/templates/default/js/lightbox/images/loading.gif "Anti-Gli1 antibody [HL247] used in Western Blot (WB). GTX635619")
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GTX635619 WB Image
Non-transfected (–) and transfected (+) RMS13 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Gli1 antibody [HL247] (GTX635619) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX635619 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Gli1 antibody [HL247] (GTX635619) diluted at 1:1000000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX635619 WB Image
Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Gli1 antibody [HL27] (GTX635619) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX635619 IHC-P Image
Gli1 antibody [HL247] detects Gli1 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse cerebellum.Gli1 stained by Gli1 antibody [HL247] (GTX635619) diluted at 1:50.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX635619 IHC-P Image
Gli1 antibody [HL27] detects Gli1 protein by immunohistochemical analysis.Sample: Paraffin-embedded rat tissues.Gli1 stained by Gli1 antibody [HL27] (GTX635619) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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REFERENCE
There are currently no references for Gli1 antibody [HL247] (GTX635619). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Gli1 antibody [HL247] (GTX635619). Be the first to share your experience with this product.
![Anti-Gli1 antibody [HL247] used in Western Blot (WB). GTX635619](/upload/website/prouct_img/small/GTX635619/GTX635619_44020_20201008_WB_shRNA_watermark_20101601_690.jpg "Anti-Gli1 antibody [HL247] used in Western Blot (WB). GTX635619")
![Anti-Gli1 antibody [HL247] used in Western Blot (WB). GTX635619](/upload/website/prouct_img/small/GTX635619/GTX635619_44020_20210924_WB_B_21100100_227.jpg "Anti-Gli1 antibody [HL247] used in Western Blot (WB). GTX635619")
![Anti-Gli1 antibody [HL247] used in Western Blot (WB). GTX635619](/upload/website/prouct_img/small/GTX635619/GTX635619_44648_20220422_WB_22080923_776.webp "Anti-Gli1 antibody [HL247] used in Western Blot (WB). GTX635619")
![Anti-Gli1 antibody [HL247] used in IHC (Paraffin sections) (IHC-P). GTX635619](/upload/website/prouct_img/small/GTX635619/GTX635619_44020_20200814_IHC-P_M_20082000_710.jpg "Anti-Gli1 antibody [HL247] used in IHC (Paraffin sections) (IHC-P). GTX635619")
![Anti-Gli1 antibody [HL247] used in IHC (Paraffin sections) (IHC-P). GTX635619](/upload/website/prouct_img/small/GTX635619/GTX635619_44648_20221227_IHC-P_multiple_R_22122821_716.webp "Anti-Gli1 antibody [HL247] used in IHC (Paraffin sections) (IHC-P). GTX635619")
![Anti-Gli1 antibody [HL247] used in Western Blot (WB). GTX635619](/upload/website/prouct_img/normal/GTX635619/GTX635619_44020_20201008_WB_shRNA_watermark_20101601_690.jpg "Anti-Gli1 antibody [HL247] used in Western Blot (WB). GTX635619")
![Anti-Gli1 antibody [HL247] used in Western Blot (WB). GTX635619](/upload/website/prouct_img/normal/GTX635619/GTX635619_44020_20210924_WB_B_21100100_227.jpg "Anti-Gli1 antibody [HL247] used in Western Blot (WB). GTX635619")
![Anti-Gli1 antibody [HL247] used in Western Blot (WB). GTX635619](/upload/website/prouct_img/normal/GTX635619/GTX635619_44648_20220422_WB_22080923_776.webp "Anti-Gli1 antibody [HL247] used in Western Blot (WB). GTX635619")
![Anti-Gli1 antibody [HL247] used in IHC (Paraffin sections) (IHC-P). GTX635619](/upload/website/prouct_img/normal/GTX635619/GTX635619_44020_20200814_IHC-P_M_20082000_710.jpg "Anti-Gli1 antibody [HL247] used in IHC (Paraffin sections) (IHC-P). GTX635619")
![Anti-Gli1 antibody [HL247] used in IHC (Paraffin sections) (IHC-P). GTX635619](/upload/website/prouct_img/normal/GTX635619/GTX635619_44648_20221227_IHC-P_multiple_R_22122821_716.webp "Anti-Gli1 antibody [HL247] used in IHC (Paraffin sections) (IHC-P). GTX635619")