GeneTex
United States (US)

Gli1 antibody

GTX106207_40030_20180112_WB_shRNA_19011506_123.jpg
GTX106207_40030_20180112_IP_19011506_736.jpg
GTX106207_40030_20180112_WB_B_19011506_388.jpg

Cat No. GTX106207

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IP
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
IP 1:1000-1:1000
Not tested in other applications.

Calculated MW

118 kDa. ( Note )

Positive Control

293T , Gli1 shRNA-transfected 293T , DDDDK-tagged Gli1-transfected 293T

Predict Reactivity

Mouse, Rat, Bovine, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GLI1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Gli Family Zinc Finger 1,Gli,Gli1

Cellular Localization

Cytoplasm , Nucleus

Background

This gene encodes a protein which is a member of the Kruppel family of zinc finger proteins. The function of this gene has not been determined; however, it may play a role in normal development gene transcription. Mouse mutation studies indicate possible involvement in human foregut malformation. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX106207_40030_20180112_WB_shRNA_19011506_123.jpg

GTX106207 WB Image

293T whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with Gli1 antibody (GTX106207) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX106207_40030_20180112_IP_19011506_736.jpg

GTX106207 IP Image

Immunoprecipitation of Gli1 protein from NT2D1 nuclear extracts using 5 µg of Gli1 antibody (GTX106207).
Western blot analysis was performed using Gli1 antibody (GTX106207).
EasyBlot HRP-conjugated anti rabbit IgG antibody (GTX221666-01) was used to detect the primary antibody.

GTX106207_40030_20180112_WB_B_19011506_388.jpg

GTX106207 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with Gli1 antibody (GTX106207) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REVIEW

Gli1 antibody Cat No. GTX106207

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 03-Jul-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : HCT116 cells
Amount used : 20μg
Blocking : 5% non-fat milk in TBST, 4°C, 1Hr
Primary Antibody : 1:1000, 4°C, 24Hr
SDS
Glycerol.pdf
Thimerosal.pdf