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Gli1 antibody

KO/KD Overexpression
Anti-Gli1 antibody used in IHC (Paraffin sections) (IHC-P). GTX106207
Anti-Gli1 antibody used in Western Blot (WB). GTX106207
Anti-Gli1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106207
Anti-Gli1 antibody used in Western Blot (WB). GTX106207
Anti-Gli1 antibody used in Immunoprecipitation (IP). GTX106207
Anti-Gli1 antibody used in Western Blot (WB). GTX106207

Cat. No. GTX106207

Reference 1
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Gli1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
IP 1:1000-1:1000
Not tested in other applications.

Calculated MW

118 kDa. ( Note )

Positive Control

DDDDK-tagged Gli1-transfected 293T , 293T

Predict Reactivity

Rat, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.12 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GLI1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037087

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

GLI family zinc finger 1 , GLI , PAPA8

Cellular Localization

Cytoplasm , Nucleus

Background

This gene encodes a protein which is a member of the Kruppel family of zinc finger proteins. The function of this gene has not been determined; however, it may play a role in normal development gene transcription. Mouse mutation studies indicate possible involvement in human foregut malformation. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Gli1 antibody used in IHC (Paraffin sections) (IHC-P). GTX106207

GTX106207 IHC-P Image

Gli1 antibody detects Gli1 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cerebellum.
Gli1 stained by Gli1 antibody (GTX106207) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Gli1 antibody used in Western Blot (WB). GTX106207

GTX106207 WB Image

RMS13 whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Gli1 antibody (GTX106207) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Gli1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106207

GTX106207 ICC/IF Image

Gli1 antibody detects Gli1 protein at nucleus by immunofluorescent analysis.
Sample: RMS-13 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Gli1 stained by Gli1 antibody (GTX106207) diluted at 1:500.

Anti-Gli1 antibody used in Western Blot (WB). GTX106207

GTX106207 WB Image

KO/KD

293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Gli1 antibody (GTX106207) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Gli1 antibody used in Immunoprecipitation (IP). GTX106207

GTX106207 IP Image

Immunoprecipitation of Gli1 protein from NT2D1 nuclear extracts using 5 μg of Gli1 antibody (GTX106207).
Western blot analysis was performed using Gli1 antibody (GTX106207) diluted at 1:500.
EasyBlot HRP-conjugated anti rabbit IgG antibody (GTX221666-01) was used to detect the primary antibody.

Anti-Gli1 antibody used in Western Blot (WB). GTX106207

GTX106207 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Gli1 antibody (GTX106207) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Liu B et al. Cell Cycle 2020; 19 (24) : 3546-3562 Promoting roles of long non-coding RNA FAM83H-AS1 in bladder cancer growth, metastasis, and angiogenesis through the c-Myc-mediated ULK3 upregulation.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

Gli1 antibody Cat. No. GTX106207

Rating
( Average 5 based on 1 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-Gli1 antibody used in Western Blot (WB). GTX106207
Date : Anonymous submitted on 03-Jul-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : HCT116 cells
Amount used : 20μg
Blocking : 5% non-fat milk in TBST, 4°C, 1Hr
Primary Antibody : 1:1000, 4°C, 24Hr
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169