Gli2 antibody, Internal

IHC-Fr analysis of mouse gut tissue using GTX46056 Gli2 antibody at 1:50.
Green : GL2
Red : Neural crest cell.
Fixed tissue was treated with 0.1% Triton X-100 for 4-6 hours at room temperature or overnight at 4ºC, washed in PBS, incubated with primary antibodies for 4-6 hours at room temperature or overnight at 4ºC, washed in PBS, and then stained with secondary antibody (Goat Anti-Rabbit Cy3) for 4-6 hours at room temperature or overnight at 4ºC.

IHC-P analysis of human heart tissue using GTX46056 Gli2 antibody at 4.0-8.0μg/ml.

WB analysis of HepG2 cells using GTX46056 Gli2 antibody at 1.0μg/ml.

WB analysis of Jurkat cells using GTX46056 Gli2 antibody at 2.5μg/ml.
Lane A : primary antibody
Lane B : primary antibody + blocking peptide

IHC-P analysis of human intestine tissue using GTX46056 Gli2 antibody at 4.0-8.0μg/ml.