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Gli2 antibody

Anti-Gli2 antibody used in IHC (Paraffin sections) (IHC-P). GTX27195
Anti-Gli2 antibody used in IHC (Paraffin sections) (IHC-P). GTX27195
Anti-Gli2 antibody used in Western Blot (WB). GTX27195
Anti-Gli2 antibody used in IHC (Paraffin sections) (IHC-P). GTX27195
Anti-Gli2 antibody used in IHC (Paraffin sections) (IHC-P). GTX27195
Anti-Gli2 antibody used in Western Blot (WB). GTX27195

Cat. No. GTX27195

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC-Fr, ELISA, IHC

Reactivity

 Human, Mouse
Package
50 μg ($399)
See all Gli2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
IHC-P 2-20 μg/mL
IHC-Fr Assay dependent
ELISA 1:15000-1:60000
IHC Assay dependent
Not tested in other applications.

Calculated MW

165 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.02 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Synthetic peptide corresponding to amino acids from an internal region of Mouse Gli-2.

Purification

Purified by antigen-affinity chromatography.
From serum

Conjugation

Unconjugated

RRID

AB_386083

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

GLI-Kruppel family member GLI2

Cellular Localization

Nucleus,Cytoplasm

Background

This protein belongs to the C2H2-type zinc finger protein subclass of the Gli family. Members of this subclass are characterized as transcription factors that bind DNA through zinc finger motifs. These motifs contain conserved H-C links. Gli family zinc finger proteins are mediators of Sonic hedgehog (Shh) signaling and they are implicated as potent oncogenes in the embryonal carcinoma cell. The protein encoded by this gene localizes to the cytoplasm and activates patched Drosophila homolog (PTCH) gene expression. Gli2 is also thought to play a role during embryogenesis. The encoded protein is associated with several phenotypes- Greig cephalopolysyndactyly syndrome, Pallister-Hall syndrome, preaxial polydactyly type IV, postaxial polydactyly types A1 and B.

Database

Research Area

DATA IMAGES

Anti-Gli2 antibody used in IHC (Paraffin sections) (IHC-P). GTX27195

GTX27195 IHC-P Image

GeneTex's Affinity Purified anti-mouse Gli-2 antibody (GTX27195) was used at 10 μg/ml to evaluate staining on several mouse tissues. Moderate to strong staining was seen on many tissues, with low background staining. This image shows Gli-2 staining of mouse testis. Tissue was formalin-fixed and paraffin embedded.

Anti-Gli2 antibody used in IHC (Paraffin sections) (IHC-P). GTX27195

GTX27195 IHC-P Image

GeneTex's Affinity Purified anti-mouse Gli-2 antibody (GTX27195) was used at 10 μg/ml to evaluate staining on several mouse tissues. Moderate to strong staining was seen on many tissues with low background staining. This image shows Gli-2 staining of mouse brain. Tissue was formalin-fixed and paraffin embedded.

Anti-Gli2 antibody used in Western Blot (WB). GTX27195

GTX27195 WB Image

Western blot using GeneTex's Affinity Purified anti-Gli-2 antibody shows detection of a predominant band at ~190 kDa corresponding to Gli-2 (arrowhead) in mouse brain whole cell lysate (lane 1). Pre-incubation of antibody with immunizing peptide completely blocks staining of this band (lane 2). ~ 25 μg of lysate was resolved on a 4-8% Tris-glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking with 5% goat serum and 0.5% BLOTTO in PBS, the membrane was probed with the primary antibody diluted to 1:750. Incubation was at room temperature for 2 h followed by washes and reaction with a 1:10,000 dilution of IRDyeR 800 conjugated Gt-a-Rabbit IgG (H&L) MX10 for 45 min at room temperature. Molecular weight markers are shown (M) using the 700 nm channel (red). IRDyeR 800 fluorescence image was captured using the OdysseyR Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

Anti-Gli2 antibody used in IHC (Paraffin sections) (IHC-P). GTX27195

GTX27195 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX27195 Gli2 antibody.
Dilution : 10 μg/mL

Anti-Gli2 antibody used in IHC (Paraffin sections) (IHC-P). GTX27195

GTX27195 IHC-P Image

IHC-P analysis of mouse testis tissue using GTX27195 Gli2 antibody.
Dilution : 10 μg/mL

Anti-Gli2 antibody used in Western Blot (WB). GTX27195

GTX27195 WB Image

WB analysis of mouse brain tissue lysate using GTX27195 Gli2 antibody prior incubated with (lane 2) or without (lane 1) blocking peptide.
Loading : 25 μg
Dilution : 1:750

REFERENCE

Application Reference

Morgan H et al. Methods Mol Biol 2018; (Epub) Identification of Human Cutaneous Basal Cell Carcinoma Cancer Stem Cells.
Application : IHC-Fr
Reactivity : Human
Ma X et al. Neuroreport 2017; 28 (3) : 141-148 Crosstalk between Notch and Sonic hedgehog signaling in a mouse model of amyotrophic lateral sclerosis.
Colmont CS et al. Proc Natl Acad Sci U S A 2013; 110 (4) : 1434-9 CD200-expressing human basal cell carcinoma cells initiate tumor growth.
Application : IHC
Reactivity : Human
Submit a Reference

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399