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Gli2a antibody

Anti-Gli2a antibody used in IHC (Whole mount) (IHC-Wm). GTX128280
Anti-Gli2a antibody used in Western Blot (WB). GTX128280

Cat. No. GTX128280

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-Wm

Reactivity

 Zebrafish, Xenopus
Package
100 μl ($399),
25 μl ($169)
See all zebrafish Gli2a products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:100-1:1000
IHC-Wm 1:50-1:500
Not tested in other applications.

Positive Control

Zebrafish 24 hpf

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Zebrafish

Immunogen

Recombinant protein encompassing a sequence within the center region of zebrafish Gli2a. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885746

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

GLI family zinc finger 2a , fc85b05 , gli2 , wu:fc85b05 , yot , you-too

Database

Research Area

DATA IMAGES

Anti-Gli2a antibody used in IHC (Whole mount) (IHC-Wm). GTX128280

GTX128280 IHC-Wm Image

Gli2a antibody detects Gli2a protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 1 day-post-fertilization zebrafish embryo.
Gli2a antibody (GTX128280) dilution: 1:50.
Anti-Gli2a antibody used in Western Blot (WB). GTX128280

GTX128280 WB Image

Gli2a antibody detects Gli2a protein (truncated repressor form, <48kDa) by Western blot analysis.
A. 60 μg Zebrafish 24 hpf lysate/extract
10 % SDS-PAGE
Gli2a antibody (GTX128280) dilution: 1:500

REFERENCE

Application Reference

Zhao Y et al. J Cell Commun Signal 2023; 17 (4) : 1335-1354 Knockdown of Yap attenuates TAA-induced hepatic fibrosis by interaction with hedgehog signals.
Belgacem YH et al. Proc Natl Acad Sci U S A 2015; 112 (13) : 818266 Inversion of Sonic hedgehog action on its canonical pathway by electrical activity.
Application : IHC-Wm
Reactivity : Xenopus
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169