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GluR1 antibody

Anti-GluR1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX132945
Anti-GluR1 antibody used in Western Blot (WB). GTX132945

Cat No. GTX132945

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-Fr
Reactivity Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

102 kDa. ( Note )

Positive Control

mouse brain , rat brain

Predict Reactivity

Human, Bovine, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.64 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human GluR1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

glutamate ionotropic receptor AMPA type subunit 1,GLUH1,GLUR1,GLURA,GluA1,HBGR1

Cellular Localization

Cell membrane,Endoplasmic reticulum membrane,Cell projection, dendrite

Background

Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes with multiple subunits, each possessing transmembrane regions, and all arranged to form a ligand-gated ion channel. The classification of glutamate receptors is based on their activation by different pharmacologic agonists. This gene belongs to a family of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) receptors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Anti-GluR1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX132945

GTX132945 IHC-Fr Image

GluR1 antibody detects GluR1 protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse mouse cerebellum.
Green: GluR1 stained by GluR1 antibody (GTX132945) diluted at 1:250.
Red: NF-H, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 10 min

Anti-GluR1 antibody used in Western Blot (WB). GTX132945

GTX132945 WB Image

Various tissue extracts (50 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GluR1 antibody (GTX132945) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169