GeneTex
United States (US)

GluR1 (phospho Ser845) antibody

GluR1-Subunit-phospho-Ser845-antibody_Western-blot_GTX30221-1_18121410_426.jpg

Cat No. GTX30221

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC
Reactivity Rat
APPLICATION

Application Note

Optimal working dilutions should be determined experimentally by the end user.

Calculated MW

100 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

100 μl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Rat

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser845 conjugated to KLH

Purification

Affinity Purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Glutamate Ionotropic Receptor Ampa Type Subunit 1,Glua1,Glur-A,Gria1

Cellular Localization

Interaction with CACNG2 promotes cell surface expression.

Background

Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist.

Database

Research Area

DATA IMAGES
GluR1-Subunit-phospho-Ser845-antibody_Western-blot_GTX30221-1_18121410_426.jpg

GTX30221 WB Image

Western blot of rat hippocampal lysate showing specific immunolabeling of the ~100k GluR1 protein phosphorylated at Ser845 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: λ-Ptase). The blot is identical to the control except that the second lane (lambda-phosphatase: λ-Ptase). The blot is identical to the control except that it was incubated in λ-Ptase (1200 units for 30 min) before being exposed to the GluR1 Ser845 antibody (GTX30221). The immunolabeling is completely eliminated by treatment with λ-Ptase.

Package List Price ($)
$ 329