GeneTex
United States (US)

GluR4 antibody, Internal

GTX88589_861_IHC-P_18122102_240.jpg
GTX88589_2149_WB_18122102_596.jpg

Cat No. GTX88589

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.3-1μg/ml
IHC-P 5-10μg/ml

Note :

IHC-P
Human Cortex shows cytoplasm staining of neuronal cells.

Not tested in other applications.

Calculated MW

101 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88589-PEP).

Specificity/Sensitivity

This antibody is expected to recognize all reported isforms (NP_000820.3; NP_001070711.1; NP_001070712.1). Reported variants NP_001070712.1 and NP_001106283.1 represent identical protein.

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-KKLDQREYPGSETP, from the internal region of the protein sequence according to NP_000820.3; NP_001070711.1; NP_001070712.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Glutamate Ionotropic Receptor Ampa Type Subunit 4,Glur4,Glur4C,Glurd,Glua4,Nedsga,Gria4

Background

Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes composed of multiple subunits, arranged to form ligand-gated ion channels. The classification of glutamate receptors is based on their activation by different pharmacologic agonists. The subunit encoded by this gene belongs to a family of AMPA (alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate)-sensitive glutamate receptors, and is subject to RNA editing (AGA->GGA; R->G). Alternative splicing of this gene results in transcript variants encoding different isoforms, which may vary in their signal transduction properties. Some haplotypes of this gene show a positive association with schizophrenia. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX88589_861_IHC-P_18122102_240.jpg

GTX88589 IHC-P Image

IHC-P analysis of human cortex using GTX88589 GluR4 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 5µg/ml

GTX88589_2149_WB_18122102_596.jpg

GTX88589 WB Image

WB analysis of human cerebellum lysate using GTX88589 GluR4 antibody, Internal.
Dilution : 0.3µg/ml
Loading : 35µg protein in RIPA buffer

Package List Price ($)
$ 319