*Optimal dilutions/concentrations should be determined by the researcher.
Human Cortex shows cytoplasm staining of neuronal cells.
Not tested in other applications.
The peptide used to generate this antibody is available for purchase (GTX88589-PEP).
This antibody is expected to recognize all reported isforms (NP_000820.3; NP_001070711.1; NP_001070712.1). Reported variants NP_001070712.1 and NP_001106283.1 represent identical protein.
Mouse, Rat, Bovine(>80% identity)
Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Peptide with sequence C-KKLDQREYPGSETP, from the internal region of the protein sequence according to NP_000820.3; NP_001070711.1; NP_001070712.1.
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Glutamate Ionotropic Receptor Ampa Type Subunit 4,Glur4,Glur4C,Glurd,Glua4,Nedsga,Gria4
Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes composed of multiple subunits, arranged to form ligand-gated ion channels. The classification of glutamate receptors is based on their activation by different pharmacologic agonists. The subunit encoded by this gene belongs to a family of AMPA (alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate)-sensitive glutamate receptors, and is subject to RNA editing (AGA->GGA; R->G). Alternative splicing of this gene results in transcript variants encoding different isoforms, which may vary in their signal transduction properties. Some haplotypes of this gene show a positive association with schizophrenia. [provided by RefSeq, Jul 2008]
GTX88589 IHC-P Image
IHC-P analysis of human cortex using GTX88589 GluR4 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 5µg/ml
GTX88589 WB Image
WB analysis of human cerebellum lysate using GTX88589 GluR4 antibody, Internal.
Dilution : 0.3µg/ml
Loading : 35µg protein in RIPA buffer