*Optimal dilutions/concentrations should be determined by the researcher.
Application
Recommended Dilution
WB
1-2μg/ml
ICC/IF
1-2μg/ml
IHC-P
1-2μg/ml for 30 minutes at RT
FACS
1-2μg/10⁶ cells
ELISA
1-2μg/ml (for coating)
Protein Array
Assay dependent
Note :
IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
ELISA
For ELISA coating, recommend using BSA-free format (please contact us for PBS only format).
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fragment of human GLUT1 protein (around aa 203-305) (exact sequence is proprietary)
Purification
Protein A/G purified
Conjugation
Unconjugated
RRID
AB_2887571
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
This gene encodes a major glucose transporter in the mammalian blood-brain barrier. The encoded protein is found primarily in the cell membrane and on the cell surface, where it can also function as a receptor for human T-cell leukemia virus (HTLV) I and II. Mutations in this gene have been found in a family with paroxysmal exertion-induced dyskinesia. [provided by RefSeq, Apr 2013]
ICC/IF analysis of K562 cells using GTX17986 GluT1 antibody [GLUT1/2475]. Green : Primary antibody Red : nucleus
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GTX17986 IHC-P Image
IHC-P analysis of human bladder carcinoma tissue using GTX17986 GluT1 antibody [GLUT1/2475].
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GTX17986 WB Image
WB analysis of A431 cell lysate using GTX17986 GluT1 antibody [GLUT1/2475].
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GTX17986 IHC-P Image
IHC-P analysis of human tongue tissue using GTX17986 GluT1 antibody [GLUT1/2475].
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GTX17986 Protein Array Image
Analysis of Protein Array containing more than 19,000 full-length human proteins using GLUT-1 Mouse Monoclonal Antibody (GLUT1/2475). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.
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GTX17986 FACS Image
FACS analysis of K562 cells using GTX17986 GluT1 antibody [GLUT1/2475]. Blue : Primary antibody Red : Isotype control