APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| Assay dependent |
| 1:2,000 |
| Assay dependent |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
human pancreas
Product Note
Monoclonal Anti-Glucagon reacts with pancreatic glucagon in RIA and immunocytochemistry. The affinity constant of 6.1 x 10(8) L/M in RIA. The antibody weakly cross-reacts with gut glucagon (enteroglucagon) in an immunohistological assay. Cross-reactivity has been observed with glucagon-containing cells in fixed sections of pancreas from human, porcine, dog, rabbit, mouse, rat, guinea pig, and cat.
PROPERTIES
Form
Liquid
Buffer
Ascites
Preservative
15mM Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Antigen Species
Pig
Immunogen
Polymerized porcine glucagon.
Purification
Unpurified
Conjugation
Unconjugated
RRID
AB_381217
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
glucagon , GLP-1 , GLP1 , GLP2 , GRPP
Cellular Localization
Secreted
Background
Glucagon is a 29-residue polypeptide hormone (MW 3482), produced in the pancreas. A related hormone, enteroglucagon (or oxyntomodulin), which is produced in the mucosa of the small and large intestine, consists of the 29 amino acid sequence of pancreatic glucagon extended by 8 additional residues at the C-terminus. The biological activities of pancreatic glucagon include glycogenolysis, lipolysis, gluconeogenesis, and ketogenesis, which are antagonistic effects to those of insulin action, thus leading to increased blood glucose levels. Immunocytochemical studies have revealed the presence of pancreatic glucagon inside the A or alpha cells, which constitute 15-20% of the islet cell population. These cells are located preferentially at the periphery of the human pancreatic islets. Pathological manifestations of the glucagon-type peptide reside almost exclusively with the existence of tumors or glucagonomas, as no states of glucagon-cell deficiency or hyperplasia have been identified. Glucagon-specific antibodies would prove useful as an cell and tumor markers applying immunohistochemical techniques, and as an analytical tool in quantification of the hormone.
Database
Research Area
REFERENCE
REVIEW
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