GeneTex
United States (US)

Glucagon antibody [K79bB10]

Cat No. GTX10988

Host Mouse
Clonality Monoclonal
Clone Name K79bB10
Isotype IgG1
Application ICC/IF, IHC-P, Dot, IHC, RIA
Reactivity Human, Mouse, Rat, Rabbit, Cat, Dog, Guinea pig, Pig
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF Assay dependent
IHC-P 1:2,000
Dot Assay dependent
IHC Assay dependent
RIA Assay dependent
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

Positive Control

human pancreas

Specificity/Sensitivity

Monoclonal Anti-Glucagon reacts with pancreatic glucagon in RIA and immunocytochemistry. The affinity constant of 6.1 x 10(8) L/M in RIA. The antibody weakly cross-reacts with gut glucagon (enteroglucagon) in an immunohistological assay. Cross-reactivity has been observed with glucagon-containing cells in fixed sections of pancreas from human, porcine, dog, rabbit, mouse, rat, guinea pig, and cat.
PROPERTIES

Form

Liquid

Buffer

Ascites, 15 mM sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Pig

Immunogen

Polymerized porcine glucagon.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Glucagon,Glp-1,Glp1,Glp2,Grpp,Gcg

Background

Glucagon is a 29-residue polypeptide hormone (MW 3482), produced in the pancreas. A related hormone, enteroglucagon (or oxyntomodulin), which is produced in the mucosa of the small and large intestine, consists of the 29 amino acid sequence of pancreatic glucagon extended by 8 additional residues at the C-terminus. The biological activities of pancreatic glucagon include glycogenolysis, lipolysis, gluconeogenesis, and ketogenesis, which are antagonistic effects to those of insulin action, thus leading to increased blood glucose levels. Immunocytochemical studies have revealed the presence of pancreatic glucagon inside the A or alpha cells, which constitute 15-20% of the islet cell population. These cells are located preferentially at the periphery of the human pancreatic islets. Pathological manifestations of the glucagon-type peptide reside almost exclusively with the existence of tumors or glucagonomas, as no states of glucagon-cell deficiency or hyperplasia have been identified. Glucagon-specific antibodies would prove useful as an cell and tumor markers applying immunohistochemical techniques, and as an analytical tool in quantification of the hormone.

Database

Research Area

REFERENCE
Package List Price ($)
$ 329