GeneTex
United States (US)

Glucocorticoid Receptor alpha antibody

GTX16318_352_ICC-IF_19011519_291.jpg
GTX16318_1052_IHC-P_19011519_995.jpg
GTX16318_1054_IHC-P_19011519_456.jpg
GTX16318_1053_IHC-P_19011519_504.jpg

Cat No. GTX16318

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, FACS, IP, ChIP assay, GSA, IHC
Reactivity Human, Mouse, Rat, Sheep
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 4 μg/ml
ICC/IF 1:100-1:200
IHC-P 1:20
FACS Assay dependent
IP Assay dependent
ChIP assay Assay dependent
GSA Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

86 kDa. ( Note )

Specificity/Sensitivity

This antibody does not detect GR beta.
PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic Peptide: C(755) E I I T N Q I P K Y S N G N I K K(771)

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Nuclear Receptor Subfamily 3 Group C Member 1,Gccr,Gcr,Gcrst,Gr,Grl,Nr3C1

Cellular Localization

Centrosome,Cytoplasm,Cytoskeleton,Microtubule organizing center,Mitochondrion,Nucleus,Spindle

Background

This gene encodes glucocorticoid receptor, which can function both as a transcription factor that binds to glucocorticoid response elements in the promoters of glucocorticoid responsive genes to activate their transcription, and as a regulator of other transcription factors. This receptor is typically found in the cytoplasm, but upon ligand binding, is transported into the nucleus. It is involved in inflammatory responses, cellular proliferation, and differentiation in target tissues. Mutations in this gene are associated with generalized glucocorticoid resistance. Alternative splicing of this gene results in transcript variants encoding either the same or different isoforms. Additional isoforms resulting from the use of alternate in-frame translation initiation sites have also been described, and shown to be functional, displaying diverse cytoplasm-to-nucleus trafficking patterns and distinct transcriptional activities (PMID:15866175). [provided by RefSeq, Feb 2011]

Database

Research Area

DATA IMAGES
GTX16318_352_ICC-IF_19011519_291.jpg

GTX16318 ICC/IF Image

ICC/IF analysis of A2058 Cells using GTX16318 Glucocorticoid Receptor alpha antibody. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4℃

GTX16318_1052_IHC-P_19011519_995.jpg

GTX16318 IHC-P Image

IHC-P analysis of human cervical carcinoma tissue using GTX16318 Glucocorticoid Receptor alpha antibody.
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:20

GTX16318_1054_IHC-P_19011519_456.jpg

GTX16318 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX16318 Glucocorticoid Receptor alpha antibody.
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:50

GTX16318_1053_IHC-P_19011519_504.jpg

GTX16318 IHC-P Image

IHC-P analysis of human heart tissue using GTX16318 Glucocorticoid Receptor alpha antibody.
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:100

Package List Price ($)
$ 319