APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 4 μg/ml |
| 1:100-1:200 |
| 1:20 |
| Assay dependent |
| Assay dependent |
| Assay dependent |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
Calculated MW
Product Note
This antibody does not detect GR beta.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Synthetic Peptide: C(755) E I I T N Q I P K Y S N G N I K K(771)
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
nuclear receptor subfamily 3 group C member 1 , GCCR , GCR , GCRST , GR , GRL
Cellular Localization
Nucleus,Mitochondrion,Cytoplasm
Background
This gene encodes glucocorticoid receptor, which can function both as a transcription factor that binds to glucocorticoid response elements in the promoters of glucocorticoid responsive genes to activate their transcription, and as a regulator of other transcription factors. This receptor is typically found in the cytoplasm, but upon ligand binding, is transported into the nucleus. It is involved in inflammatory responses, cellular proliferation, and differentiation in target tissues. Mutations in this gene are associated with generalized glucocorticoid resistance. Alternative splicing of this gene results in transcript variants encoding either the same or different isoforms. Additional isoforms resulting from the use of alternate in-frame translation initiation sites have also been described, and shown to be functional, displaying diverse cytoplasm-to-nucleus trafficking patterns and distinct transcriptional activities (PMID:15866175). [provided by RefSeq, Feb 2011]
Database
Research Area
DATA IMAGES
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GTX16318 IHC-P Image
IHC-P analysis of human cervical carcinoma tissue using GTX16318 Glucocorticoid Receptor alpha antibody.
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:20
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GTX16318 ICC/IF Image
ICC/IF analysis of A2058 Cells using GTX16318 Glucocorticoid Receptor alpha antibody. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4ºC
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GTX16318 IHC-P Image
IHC-P analysis of human tonsil tissue using GTX16318 Glucocorticoid Receptor alpha antibody.
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:50
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GTX16318 IHC-P Image
IHC-P analysis of human heart tissue using GTX16318 Glucocorticoid Receptor alpha antibody.
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:100
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REFERENCE
There are currently no references for Glucocorticoid Receptor alpha antibody (GTX16318). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Glucocorticoid Receptor alpha antibody (GTX16318). Be the first to share your experience with this product.
