Glucocorticoid Receptor antibody [6E6]

Cat. No. GTX60389

Host	Mouse
Clonality	Monoclonal
Clone Name	6E6
Isotype	IgG1
Application	WB, ICC/IF, IHC-P, FACS, ELISA
Reactivity	Human
Package	100 μl ($409)

See all Glucocorticoid Receptor products

Datasheet
Document

View product citations for antibody GTX60389 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1/500 - 1/2000
ICC/IF	1/200 - 1/1000
IHC-P	1/200 - 1/1000
FACS	1/200 - 1/400
ELISA	1/10000

Not tested in other applications.

Calculated MW

86 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human NR3C1 expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nuclear receptor subfamily 3 group C member 1 , GCCR , GCR , GCRST , GR , GRL

Cellular Localization

Nucleus,Mitochondrion,Cytoplasm

Background

This gene encodes glucocorticoid receptor, which can function both as a transcription factor that binds to glucocorticoid response elements in the promoters of glucocorticoid responsive genes to activate their transcription, and as a regulator of other transcription factors. This receptor is typically found in the cytoplasm, but upon ligand binding, is transported into the nucleus. It is involved in inflammatory responses, cellular proliferation, and differentiation in target tissues. Mutations in this gene are associated with generalized glucocorticoid resistance. Alternative splicing of this gene results in transcript variants encoding either the same or different isoforms. Additional isoforms resulting from the use of alternate in-frame translation initiation sites have also been described, and shown to be functional, displaying diverse cytoplasm-to-nucleus trafficking patterns and distinct transcriptional activities (PMID:15866175). [provided by RefSeq, Feb 2011]

Database

Gene ID: 2908 NR3C1
UniProt: P04150 NR3C1

Research Area

DATA IMAGES

GTX60389 IHC-P Image

IHC-P analysis of lung cancer tissue using GTX60389 Glucocorticoid Receptor antibody [6E6].

GTX60389 FACS Image

FACS analysis of K562 cells using GTX60389 Glucocorticoid Receptor antibody [6E6].
Green : Glucocorticoid Receptor
Purple : negative control

GTX60389 WB Image

WB analysis of HeLa cell lysate using GTX60389 Glucocorticoid Receptor antibody [6E6].

GTX60389 ICC/IF Image

ICC/IF analysis of PC-2 cells using GTX60389 Glucocorticoid Receptor antibody [6E6].
Green : Glucocorticoid Receptor
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments

GTX60389 ELISA Image

ELISA analysis of antigen using GTX60389 Glucocorticoid Receptor antibody [6E6].
Red : Control antigen 100ng
Purple : Antigen 10ng
Green : Antigen 50ng
Blue : Antigen 100ng

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 409

Qty