Wild-type (WT) and NR3C1 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glucocorticoid Receptor antibody [HL1149] (GTX636420) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Glucocorticoid Receptor antibody [HL1149] (GTX636420) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Glucocorticoid Receptor antibody [HL1149] detects Glucocorticoid Receptor protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
Glucocorticoid Receptor stained by Glucocorticoid Receptor antibody [HL1149] (GTX636420) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glucocorticoid Receptor antibody [HL1149] (GTX636420) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Glucocorticoid Receptor antibody [HL1149] detects Glucocorticoid Receptor protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Glucocorticoid Receptor stained by Glucocorticoid Receptor antibody [HL1149] (GTX636420) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.

Glucocorticoid Receptor antibody [HL1149] detects Glucocorticoid Receptor protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
Glucocorticoid Receptor stained by Glucocorticoid Receptor antibody [HL1149] (GTX636420) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min