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Glucocorticoid Receptor antibody [HL1149]

Recombinant antibody KO/KD Orthogonal
Anti-Glucocorticoid Receptor antibody [HL1149] used in Western Blot (WB). GTX636420
Anti-Glucocorticoid Receptor antibody [HL1149] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636420
Anti-Glucocorticoid Receptor antibody [HL1149] used in Western Blot (WB). GTX636420
Anti-Glucocorticoid Receptor antibody [HL1149] used in IHC (Paraffin sections) (IHC-P). GTX636420
Anti-Glucocorticoid Receptor antibody [HL1149] used in Western Blot (WB). GTX636420
Anti-Glucocorticoid Receptor antibody [HL1149] used in IHC (Paraffin sections) (IHC-P). GTX636420

Cat. No. GTX636420

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1149

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Glucocorticoid Receptor products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

86 kDa. ( Note )

Predict Reactivity

Rat, Rabbit, Sheep, Bovine, Cat, Dog, Guinea pig, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Glucocorticoid Receptor. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2909980

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nuclear receptor subfamily 3 group C member 1 , GCCR , GCR , GCRST , GR , GRL

Cellular Localization

Nucleus,Mitochondrion,Cytoplasm

Background

This gene encodes glucocorticoid receptor, which can function both as a transcription factor that binds to glucocorticoid response elements in the promoters of glucocorticoid responsive genes to activate their transcription, and as a regulator of other transcription factors. This receptor is typically found in the cytoplasm, but upon ligand binding, is transported into the nucleus. It is involved in inflammatory responses, cellular proliferation, and differentiation in target tissues. Mutations in this gene are associated with generalized glucocorticoid resistance. Alternative splicing of this gene results in transcript variants encoding either the same or different isoforms. Additional isoforms resulting from the use of alternate in-frame translation initiation sites have also been described, and shown to be functional, displaying diverse cytoplasm-to-nucleus trafficking patterns and distinct transcriptional activities (PMID:15866175). [provided by RefSeq, Feb 2011]

Database

Research Area

DATA IMAGES

Anti-Glucocorticoid Receptor antibody [HL1149] used in Western Blot (WB). GTX636420

GTX636420 WB Image

KO/KD

Wild-type (WT) and NR3C1 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glucocorticoid Receptor antibody [HL1149] (GTX636420) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Glucocorticoid Receptor antibody [HL1149] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636420

GTX636420 ICC/IF Image

Glucocorticoid Receptor antibody [HL1149] detects Glucocorticoid Receptor protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Glucocorticoid Receptor stained by Glucocorticoid Receptor antibody [HL1149] (GTX636420) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.

Anti-Glucocorticoid Receptor antibody [HL1149] used in Western Blot (WB). GTX636420

GTX636420 WB Image

Orthogonal

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glucocorticoid Receptor antibody [HL1149] (GTX636420) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Glucocorticoid Receptor antibody [HL1149] used in IHC (Paraffin sections) (IHC-P). GTX636420

GTX636420 IHC-P Image

Glucocorticoid Receptor antibody [HL1149] detects Glucocorticoid Receptor protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
Glucocorticoid Receptor stained by Glucocorticoid Receptor antibody [HL1149] (GTX636420) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Glucocorticoid Receptor antibody [HL1149] used in Western Blot (WB). GTX636420

GTX636420 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Glucocorticoid Receptor antibody [HL1149] (GTX636420) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Glucocorticoid Receptor antibody [HL1149] used in IHC (Paraffin sections) (IHC-P). GTX636420

GTX636420 IHC-P Image

Glucocorticoid Receptor antibody [HL1149] detects Glucocorticoid Receptor protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
Glucocorticoid Receptor stained by Glucocorticoid Receptor antibody [HL1149] (GTX636420) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations