GeneTex
United States (US)

Glucocorticoid Receptor antibody

KO/KD
GTX101120_39834_IP_19011506_942.jpg
GTX101120_39834_20170323_WB_KO_watermark_19011506_735.jpg
GTX101120_WB_19011506_247.jpg
GTX101120_39834_IHC_19011506_790.jpg
GTX101120_39834_20151029_WB_R_heart_19011506_936.jpg
GTX101120_39834_20151029_WB_M_heart_19011506_655.jpg

Cat No. GTX101120

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
IP 1:500-1:1000
Not tested in other applications.

Calculated MW

86 kDa. ( Note )

Positive Control

HeLa , A431 , Mouse heart , Rat heart

Predict Reactivity

Sheep, Bovine, Cat, Dog, Guinea pig, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human Glucocorticoid Receptor. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Nuclear Receptor Subfamily 3 Group C Member 1,Gccr,Gcr,Gcrst,Gr,Grl,Nr3C1

Cellular Localization

Cytoplasm , Nucleus

Background

The protein encoded by this gene is a receptor for glucocorticoids that can act as both a transcription factor and as a regulator of other transcription factors. This protein can also be found in heteromeric cytoplasmic complexes along with heat shock factors and immunophilins. The protein is typically found in the cytoplasm until it binds a ligand, which induces transport into the nucleus. Mutations in this gene are a cause of glucocorticoid resistance, or cortisol, resistance. Alternate splicing, the use of at least three different promoters, and alternate translation initiation sites result in several transcript variants encoding the same protein or different isoforms, but the full-length nature of some variants has not been determined. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101120_39834_IP_19011506_942.jpg

GTX101120 IP Image

Glucocorticoid receptor antibody immunoprecipitates Glucocorticoid receptor protein in IP experiments. IP Sample: 1000 µg HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2.5 μg of preimmune rabbit IgG C. Immunoprecipitation of Glucocorticoid receptor protein by 2.5 μg of Glucocorticoid receptor antibody (GTX101120) 7.5% SDS-PAGE The immunoprecipitated Glucocorticoid receptor protein was detected by SCMH1 antibody (GTX101120) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX101120_39834_20170323_WB_KO_watermark_19011506_735.jpg

GTX101120 WB Image

KO/KD

Wild-type (WT) and Glucocorticoid receptor knockout (KO) HeLa cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glucocorticoid receptor antibody (GTX101120) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
CRISPR knockout cell lines generated by www.edigene.com

GTX101120_WB_19011506_247.jpg

GTX101120 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
7.5% SDS PAGE
GTX101120 diluted at 1:1000

GTX101120_39834_IHC_19011506_790.jpg

GTX101120 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Hela xenograft, using Glucocorticoid receptor(GTX101120) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101120_39834_20151029_WB_R_heart_19011506_936.jpg

GTX101120 WB Image

Glucocorticoid receptor antibody detects Glucocorticoid receptor protein by western blot analysis. Rat tissue extracts (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Glucocorticoid receptor antibody (GTX101120) diluted at 1:1000.

GTX101120_39834_20151029_WB_M_heart_19011506_655.jpg

GTX101120 WB Image

Glucocorticoid receptor antibody detects Glucocorticoid receptor protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Glucocorticoid receptor antibody (GTX101120) diluted at 1:1000.

REFERENCE
REVIEW

Glucocorticoid Receptor antibody Cat No. GTX101120

Rating ( Average 4 based on 1 users reviews)
Application
Western Blot(WB) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 08-Nov-2017
Score :
Application Tested : WB
Sample Species : Hu
Sample : HaCat cells
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, overnightHr
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169