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Glucocorticoid Receptor antibody

KO/KD
Anti-Glucocorticoid Receptor antibody used in Western Blot (WB). GTX101120
Anti-Glucocorticoid Receptor antibody used in Immunoprecipitation (IP). GTX101120
Anti-Glucocorticoid Receptor antibody used in IHC (Paraffin sections) (IHC-P). GTX101120
Anti-Glucocorticoid Receptor antibody used in Western Blot (WB). GTX101120
Anti-Glucocorticoid Receptor antibody used in Western Blot (WB). GTX101120
Anti-Glucocorticoid Receptor antibody used in Western Blot (WB). GTX101120

Cat. No. GTX101120

Reference 15
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Glucocorticoid Receptor products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
IP 1:500-1:1000
Not tested in other applications.

Calculated MW

86 kDa. ( Note )

Positive Control

A431 , mouse heart , rat heart , HeLa

Predict Reactivity

Sheep, Bovine, Cat, Dog, Guinea pig, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human Glucocorticoid Receptor. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951023

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nuclear receptor subfamily 3 group C member 1 , GCCR , GCR , GCRST , GR , GRL

Cellular Localization

Cytoplasm , Nucleus

Background

The protein encoded by this gene is a receptor for glucocorticoids that can act as both a transcription factor and as a regulator of other transcription factors. This protein can also be found in heteromeric cytoplasmic complexes along with heat shock factors and immunophilins. The protein is typically found in the cytoplasm until it binds a ligand, which induces transport into the nucleus. Mutations in this gene are a cause of glucocorticoid resistance, or cortisol, resistance. Alternate splicing, the use of at least three different promoters, and alternate translation initiation sites result in several transcript variants encoding the same protein or different isoforms, but the full-length nature of some variants has not been determined. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Glucocorticoid Receptor antibody used in Western Blot (WB). GTX101120

GTX101120 WB Image

KO/KD

Wild-type (WT) and Glucocorticoid receptor knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glucocorticoid receptor antibody (GTX101120) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Glucocorticoid Receptor antibody used in Immunoprecipitation (IP). GTX101120

GTX101120 IP Image

Glucocorticoid receptor antibody immunoprecipitates Glucocorticoid receptor protein in IP experiments. IP Sample: 1000 μg HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2.5 μg of preimmune rabbit IgG C. Immunoprecipitation of Glucocorticoid receptor protein by 2.5 μg of Glucocorticoid receptor antibody (GTX101120) 7.5% SDS-PAGE The immunoprecipitated Glucocorticoid receptor protein was detected by SCMH1 antibody (GTX101120) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-Glucocorticoid Receptor antibody used in IHC (Paraffin sections) (IHC-P). GTX101120

GTX101120 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Hela xenograft, using Glucocorticoid receptor(GTX101120) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Glucocorticoid Receptor antibody used in Western Blot (WB). GTX101120

GTX101120 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
7.5% SDS PAGE
GTX101120 diluted at 1:1000

Anti-Glucocorticoid Receptor antibody used in Western Blot (WB). GTX101120

GTX101120 WB Image

Glucocorticoid receptor antibody detects Glucocorticoid receptor protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Glucocorticoid receptor antibody (GTX101120) diluted at 1:1000.

Anti-Glucocorticoid Receptor antibody used in Western Blot (WB). GTX101120

GTX101120 WB Image

Glucocorticoid receptor antibody detects Glucocorticoid receptor protein by western blot analysis. Rat tissue extracts (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Glucocorticoid receptor antibody (GTX101120) diluted at 1:1000.

REFERENCE

Application Reference

De Santa F et al. Microbiome 2024; 12 (1) : 29 Effect of a multi-strain probiotic mixture consumption on anxiety and depression symptoms induced in adult mice by postnatal maternal separation.
Kunke M et al. Cell Death Dis 2023; 14 (8) : 562 Targeted deletion of von-Hippel-Lindau in the proximal tubule conditions the kidney against early diabetic kidney disease.
Butz H et al. Cells 2023; 12 (5) Context-Dependent Role of Glucocorticoid Receptor Alpha and Beta in Breast Cancer Cell Behaviour.
Application : WB, IHC-P
Reactivity : Human
Hsiu-Hui Yang et al. Front Physiol 2022; 13 : 816959 Glucocorticoid Receptor Maintains Vasopressin Responses in Kidney Collecting Duct Cells.
Application : WB
Shanyong Yi et al. Anal Cell Pathol (Amst) 2021; 2021 : 5565671 Endoplasmic Reticulum Stress Is Involved in Glucocorticoid-Induced Apoptosis in PC12 Cells.
Application : ICC/IF
Reactivity : Rat
Show More +
Submit a Reference

REVIEW

Glucocorticoid Receptor antibody Cat. No. GTX101120

Rating
( Average 4 based on 1 users reviews)
Application
Western Blot(WB)
( Average 4 based on 1 users reviews)
Anti-Glucocorticoid Receptor antibody used in Western Blot (WB). GTX101120
Date : Anonymous submitted on 08-Nov-2017
Score :
Application Tested : WB
Sample Species : Hu
Sample : HaCat cells
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Submit a Review
Application Reference
De Santa F et al. Microbiome 2024; 12 (1):29 Effect of a multi-strain probiotic mixture consumption on anxiety and depression symptoms induced in adult mice by postnatal maternal separation.
Kunke M et al. Cell Death Dis 2023; 14 (8):562 Targeted deletion of von-Hippel-Lindau in the proximal tubule conditions the kidney against early diabetic kidney disease.
Butz H et al. Cells 2023; 12 (5) Context-Dependent Role of Glucocorticoid Receptor Alpha and Beta in Breast Cancer Cell Behaviour.
Application : WB, IHC-P
Reactivity : Human
Hsiu-Hui Yang et al. Front Physiol 2022; 13:816959 Glucocorticoid Receptor Maintains Vasopressin Responses in Kidney Collecting Duct Cells.
Application : WB
Shanyong Yi et al. Anal Cell Pathol (Amst) 2021; 2021:5565671 Endoplasmic Reticulum Stress Is Involved in Glucocorticoid-Induced Apoptosis in PC12 Cells.
Application : ICC/IF
Reactivity : Rat
Ho CH et al. Front Physiol 2021; 12:725172 α-Actinin 4 Links Vasopressin Short-Term and Long-Term Regulation of Aquaporin-2 in Kidney Collecting Duct Cells.
Application : WB, ICC/IF
Sueyasu T et al. Eur Rev Med Pharmacol Sci 2020; 24 (4):2110-2119 Dietary arachidonic acid improves age-related excessive enhancement of the stress response.
Application : WB
Reactivity : Mouse
Matsuzaki K et al. Int J Mol Sci 2020; 21 (3) Salivary Immunoglobulin A Secretion and Polymeric Ig Receptor Expression in the Submandibular Glands Are Enhanced in Heat-Acclimated Rats.
Application : WB
Reactivity : Rat
Obradovi? MMS et al. Nature 2019; 567 (7749):540-544 Glucocorticoids promote breast cancer metastasis.
Application : WB
Reactivity : Mouse
Kim SR et al. Antiviral Res 2018; 151:87-96 Antiviral and anti-inflammatory activity of * against human rhinovirus infection mediated via autophagy activation.
Application : WB
Reactivity : Mouse
Wu YL et al. Free Radic Biol Med 2018; 115:309-317 Caffeic acid and resveratrol ameliorate cellular damage in cell and Drosophila models of spinocerebellar ataxia type 3 through upregulation of Nrf2 pathway.
Application : WB
Reactivity : Human
Choi EJ et al. Oncogene 2017; (Epub) A clinical drug library screen identifies * propionate as an NRF2 inhibitor with potential therapeutic efficacy in KEAP1 mutant lung cancer.
Application : WB
Reactivity : Human
Bolt MJ et al. Oncogene 2015; 34 (2):154-64 Systems level-based RNAi screening by high content analysis identifies UBR5 as a regulator of estrogen receptor-α protein levels and activity.
Application : WB
Reactivity : Human
Yin Y et al. Neurobiol Dis 2013; 59:165-76 Glucocorticoid receptor β regulates injury-mediated astrocyte activation and contributes to glioma pathogenesis via modulation of β-catenin/TCF transcriptional activity.
Application : WB, ICC/IF
Reactivity : Human
Bolt MJ et al. Nucleic Acids Res 2013; 41 (7):4036-48 Coactivators enable glucocorticoid receptor recruitment to fine-tune estrogen receptor transcriptional responses.
Application : ICC/IF
Reactivity : Human
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169