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Glutamate Dehydrogenase antibody

Anti-Glutamate Dehydrogenase antibody used in Western Blot (WB). GTX105765
Anti-Glutamate Dehydrogenase antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105765
Anti-Glutamate Dehydrogenase antibody used in IHC (Paraffin sections) (IHC-P). GTX105765
Anti-Glutamate Dehydrogenase antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105765
Anti-Glutamate Dehydrogenase antibody used in IHC (Paraffin sections) (IHC-P). GTX105765

Cat No. GTX105765

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, ELISA
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
ELISA Assay dependent
Not tested in other applications.

Calculated MW

61 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , *THP1 , *Raw264.7

Predict Reactivity

Sheep, Bovine, Pig, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.07 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Glutamate Dehydrogenase. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

glutamate dehydrogenase 1 , GDH , GDH1 , GLUD

Cellular Localization

Mitochondrion matrix

Background

L-glutamate dehydrogenase (EC 1.4.1.3) has a central role in nitrogen metabolism in plants and animals. Glutamate dehydrogenase is found in all organisms and catalyzes the oxidative deamination of 1-glutamate to 2-oxoglutarate (Smith et al., 2001 [PubMed 11254391]). Glutamate, the main substrate of GLUD, is present in brain in concentrations higher than in other organs. In nervous tissue, GLUD appears to function in both the synthesis and the catabolism of glutamate and perhaps in ammonia detoxification (Mavrothalassitis et al., 1988 [PubMed 3368458]).[supplied by OMIM]

Database

Research Area

DATA IMAGES
Anti-Glutamate Dehydrogenase antibody used in Western Blot (WB). GTX105765

GTX105765 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamate Dehydrogenase antibody (GTX105765) diluted at 1:1000.

Anti-Glutamate Dehydrogenase antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105765

GTX105765 ICC/IF Image

Glutamate Dehydrogenase antibody detects Glutamate Dehydrogenase protein at cytoplasm by immunofluorescent analysis.
Sample: U-87 MG cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Glutamate Dehydrogenase protein stained by Glutamate Dehydrogenase antibody (GTX105765) diluted at 1:500.
Red: beta Tubulin 3/ TUJ1 protein stained by beta Tubulin 3/ TUJ1 antibody (GTX631836) diluted at 1:200.
Blue: Hoechst 33342 staining.

Anti-Glutamate Dehydrogenase antibody used in IHC (Paraffin sections) (IHC-P). GTX105765

GTX105765 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using Glutamate Dehydrogenase(GTX105765) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Glutamate Dehydrogenase antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105765

GTX105765 ICC/IF Image

Glutamate Dehydrogenase antibody detects Glutamate Dehydrogenase protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Glutamate Dehydrogenase protein stained by Glutamate Dehydrogenase antibody (GTX105765) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-Glutamate Dehydrogenase antibody used in IHC (Paraffin sections) (IHC-P). GTX105765

GTX105765 IHC-P Image

Glutamate Dehydrogenase antibody detects Glutamate Dehydrogenase protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Glutamate Dehydrogenase antibody (GTX105765) diluted at 1:750.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE
REVIEW

Glutamate Dehydrogenase antibody Cat No. GTX105765

Rating ( Average 4 based on 2 users reviews)
Application
Western Blot(WB) ( Average 4 based on 2 users reviews)
Date : Anonymous submitted on 25-Jun-2013
Score :
Application Tested : WB
Sample Species : Ms
Sample : Raw264.7 cells
Amount used : 45
Blocking : 5% milk, 25°C, 0.5Hr
Primary Antibody : 1:2000, 4°C, 16Hr
Date : Anonymous submitted on 25-Jun-2013
Score :
Application Tested : WB
Sample Species : Hu
Sample : THP1 cells
Amount used : 45
Blocking : 5% milk, 25°C, 0.5Hr
Primary Antibody : 1:2000, 4°C, 16Hr
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169