Glutaminase C (GAC) antibody [GT1075]

Cat. No. GTX632265

Host	Mouse
Clonality	Monoclonal
Clone Name	GT1075
Isotype	IgG2b
Application	WB, ICC/IF
Reactivity	Human
Package	100 μl ($399), 25 μl ($169)

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Datasheet
Document

View product citations for antibody GTX632265 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000

Not tested in other applications.

Calculated MW

65 kDa. ( Note )

Positive Control

MCF-7 , MDA-MB-231

Product Note

Specific for GAC but not KGA isoform

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Glutaminase C (GAC). The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888301

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glutaminase , AAD20 , CASGID , EIEE71 , GAC , GAM , GLS1 , KGA

Cellular Localization

Mitochondrion

Background

Sahai (1983) [PubMed 6825316] demonstrated phosphate-activated glutaminase (EC 3.5.1.2) in human platelets. It is the major enzyme yielding glutamate from glutamine. Significance of the enzyme derives from its possible implication in behavior disturbances in which glutamate acts as a neurotransmitter (Prusiner, 1981). High heritability of platelet glutaminase was indicated by studies of Sahai and Vogel (1983) [PubMed 6682827] who found an intraclass correlation coefficient of 0.96 for monozygotic twins and 0.53 for dizygotic twins.[supplied by OMIM]

Database

Gene ID: 2744 GLS
UniProt: O94925 GLS

Research Area

DATA IMAGES

GTX632265 ICC/IF Image

Glutaminase C antibody [GT1075] detects Glutaminase C protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Glutaminase C protein stained by Glutaminase C antibody [GT1075] (GTX632265) diluted at 1:400.
Blue: Hoechst 33342 staining.

GTX632265 WB Image

Glutaminase C (GAC) antibody detects Glutaminase C (GAC) protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glutaminase C (GAC) antibody (GTX632265) diluted at a dilution of 1:1000.

REFERENCE

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SDS
PBS.pdf

Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty Custom Conjugations