Glutaminase C (GAC) antibody

Name: Glutaminase C (GAC) antibody
Brand: GeneTex
SKU: GTX131263
Price: 399.00 USD
Rating: 4 (1 reviews)

Anti-Glutaminase C (GAC) antibody used in Western Blot (WB). GTX131263

Anti-Glutaminase C (GAC) antibody used in Immunoprecipitation (IP). GTX131263

Anti-Glutaminase C (GAC) antibody used in IHC (Paraffin sections) (IHC-P). GTX131263

Anti-Glutaminase C (GAC) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX131263

Cat. No. GTX131263

Reference 4

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IP
Reactivity	Human, Mouse
Package	100 μl ($399), 25 μl ($169)

See all Glutaminase C products

View product citations for antibody GTX131263 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:1000-1:10000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IP	1:100-1:500

Not tested in other applications.

Calculated MW

65 kDa. ( Note )

Positive Control

MDA-MB-231

Product Note

Specific for GAC but not KGA isoform

Predict Reactivity

Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Glutaminase C (GAC). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886452

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glutaminase , AAD20 , CASGID , EIEE71 , GAC , GAM , GLS1 , KGA

Cellular Localization

Mitochondrion

Background

Sahai (1983) [PubMed 6825316] demonstrated phosphate-activated glutaminase (EC 3.5.1.2) in human platelets. It is the major enzyme yielding glutamate from glutamine. Significance of the enzyme derives from its possible implication in behavior disturbances in which glutamate acts as a neurotransmitter (Prusiner, 1981). High heritability of platelet glutaminase was indicated by studies of Sahai and Vogel (1983) [PubMed 6682827] who found an intraclass correlation coefficient of 0.96 for monozygotic twins and 0.53 for dizygotic twins.[supplied by OMIM]

Database

Gene ID: 2744 GLS
UniProt: O94925 GLS

Research Area

DATA IMAGES

GTX131263 WB Image

Glutaminase C (GAC) antibody validation by shRNA knock-down. Non-transfected (-) and GLS1(GAC isoform specific) shRNA-transfected MDA-MB-231 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glutaminase C (GAC) antibody (GTX131263) at a dilution of 1:5000.
This antibody specifically recognizes GAC but not the KGA isoform

GTX131263 IP Image

Immunoprecipitation of Glutaminase C protein from MDA-MB-231 whole cell extracts using 5 μg of Glutaminase C antibody (GTX131263) or Glutaminase C antibody (GTX132402).
Western blot analysis was performed using Glutaminase C antibody (GTX131263).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX131263 IHC-P Image

Glutaminase C antibody detects Glutaminase C protein at cytoplasm on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human colon carcinoma.
Glutaminase C antibody (GTX131263) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX131263 IHC-P Image

Glutaminase C antibody detects Glutaminase C protein at cytoplasm on mouse duidenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duidenum.
Glutaminase C antibody (GTX131263) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX131263 IHC-P Image

Glutaminase C antibody detects Glutaminase C protein at cytoplasm on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Glutaminase C antibody (GTX131263) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX131263 ICC/IF Image

Glutaminase C antibody detects Glutaminase C protein at mitochondria by immunofluorescent analysis.
Sample: A549 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Glutaminase C protein stained by Glutaminase C antibody (GTX131263) diluted at 1:500.
Red: Histone H3K9ac (acetyl Lys9), a nucleus marker, stained by Histone H3K9ac (acetyl Lys9) antibody [GT464] (GTX630554) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

REFERENCE

Application Reference

Fazal S et al. Hum Mol Genet 2023; 32 (1) : 46-54 Repeat expansions nested within tandem CNVs: a unique structural change in GLS exemplifies the diagnostic challenges of non-coding pathogenic variation.

Barreca F et al. Antioxidants (Basel) 2023; 12 (8) SIRT5 Activation and Inorganic Phosphate Binding Reduce Cancer Cell Vitality by Modulating Autophagy/Mitophagy and ROS.

F. Barreca et al. Preprints.org 2023; : 2023052144 SIRT5 activation and inorganic phosphate binding reduce can- 2 cer cells vitality by modulating autophagy/mitophagy and 3 ROS.

Application : ICC/IF

Reactivity : Human

Saladini S et al. Cells 2019; 8 (1)

Application : WB

Reactivity : Human

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