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Glutaminase (KGA) antibody, C-term

Anti-Glutaminase (KGA) antibody, C-term used in Western Blot (WB). GTX50891
Anti-Glutaminase (KGA) antibody, C-term used in Western Blot (WB). GTX50891

Cat No. GTX50891

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB

Reactivity

Human, Rat, Pig
Package
50 μg ($359)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.2-2.5 ug/ml
Not tested in other applications.

Calculated MW

73 kDa. ( Note )

Positive Control

ACHN , kidney proximal tubules from cortex , LLCPK1-F+pig kidney proximal tubule , rat brain , rat kidney cortex

Specificity/Sensitivity

Specific for KGA but not GAC isoform

PROPERTIES

Form

Liquid

Buffer

1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5-1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a C-terminal region of Human GLS

Purification

Affinity Purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

glutaminase , AAD20 , CASGID , EIEE71 , GAC , GAM , GLS1 , KGA

Background

This gene encodes the K-type mitochondrial glutaminase. The encoded protein is an phosphate-activated amidohydrolase that catalyzes the hydrolysis of glutamine to glutamate and ammonia. This protein is primarily expressed in the brain and kidney plays an essential role in generating energy for metabolism, synthesizing the brain neurotransmitter glutamate and maintaining acid-base balance in the kidney. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012]

Database

Research Area

DATA IMAGES

Anti-Glutaminase (KGA) antibody, C-term used in Western Blot (WB). GTX50891

GTX50891 WB Image

WB analysis of ACHN cells using GTX50891 GLS antibody at 0.2-1μg/ml.

Anti-Glutaminase (KGA) antibody, C-term used in Western Blot (WB). GTX50891

GTX50891 WB Image

WB analysis of rat kidney cortex, kidney proximal tubules from cortex, rat brain tissue, and pig LLCPK1-F cells using GTX50891 GLS antibody at 1:2000.
Lane 5 : rat kidney cortex
Lane 6 : rat kidney proximal tubules from cortex
Lane 7 : pig LLCPK1-F cells
Lane 8 : rat brain

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf