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Glutaminase antibody, C-term

Anti-Glutaminase antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX81012
Anti-Glutaminase antibody, C-term used in Western Blot (WB). GTX81012
Anti-Glutaminase antibody, C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX81012
Anti-Glutaminase antibody, C-term used in Western Blot (WB). GTX81012
Anti-Glutaminase antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX81012
Anti-Glutaminase antibody, C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX81012
Anti-Glutaminase antibody, C-term used in Flow cytometry (FACS). GTX81012
Anti-Glutaminase antibody, C-term used in (). GTX81012

Cat. No. GTX81012

Reference 6

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human, Mouse, Rat
Package
200 μl ($399)
See all Glutaminase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ICC/IF 1:25
IHC-P 1:25
FACS 1:25
Not tested in other applications.

Calculated MW

73 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH conjugated synthetic peptide between 516-545 amino acids from the C-terminal region of human GLS.

Purification

Protein A purified, followed by peptide affinity purification.

Conjugation

Unconjugated

RRID

AB_11162809

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glutaminase , AAD20 , CASGID , EIEE71 , GAC , GAM , GLS1 , KGA

Background

This gene encodes the K-type mitochondrial glutaminase. The encoded protein is an phosphate-activated amidohydrolase that catalyzes the hydrolysis of glutamine to glutamate and ammonia. This protein is primarily expressed in the brain and kidney plays an essential role in generating energy for metabolism, synthesizing the brain neurotransmitter glutamate and maintaining acid-base balance in the kidney. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012]

Database

Research Area

DATA IMAGES

Anti-Glutaminase antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX81012

GTX81012 IHC-P Image

IHC-P analysis of human brain tissue using GTX81012 GLS antibody, C-term.
Antigen retrieval : heat-induced antigen retrieval with a citrate buffer (pH6)
Dilution : 1:25

Anti-Glutaminase antibody, C-term used in Western Blot (WB). GTX81012

GTX81012 WB Image

WB analysis of various samples using GTX81012 GLS antibody, C-term.
Lane 1: human brain lysate
Lane 2: mouse brain lysate
Lane 3: 293T/17 whole cell lysate
Loading : 20 μg per lane
Dilution : 1:2000

Anti-Glutaminase antibody, C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX81012

GTX81012 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX81012 GLS antibody, C-term.
Green : GLS
Red : Actin filament
Blue : cell nucleus
Dilution : 1:25

Anti-Glutaminase antibody, C-term used in Western Blot (WB). GTX81012

GTX81012 WB Image

WB analysis of various samples using GTX81012 GLS antibody, C-term.
Lane 1: human brain lysate
Lane 2: human kidney lysate
Lane 3: mouse brain lysate
Loading : 20 μg per lane
Dilution : 1:2000

Anti-Glutaminase antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX81012

GTX81012 IHC-P Image

IHC-P analysis of human kidney tissue using GTX81012 GLS antibody, C-term.
Antigen retrieval : heat-induced antigen retrieval with a citrate buffer (pH6)
Dilution : 1:25

Anti-Glutaminase antibody, C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX81012

GTX81012 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX81012 GLS antibody, C-term.
Green : GLS
Red : Actin filament
Blue : cell nucleus
Dilution : 1:25

Anti-Glutaminase antibody, C-term used in Flow cytometry (FACS). GTX81012

GTX81012 FACS Image

FACS analysis of U-2 OS cells using GTX81012 GLS antibody, C-term.
Green : GLS
Blue : Isotype control
Fixation : 2% paraformaldehyde (PFA) (10 min)
Permeabilization : 90% methanol (10 min)
Dilution : 1:25

Anti-Glutaminase antibody, C-term used in (). GTX81012

GTX81012 Image

The data was published in the 2022 in Int J Mol Sci. PMID: 35054778

REFERENCE

Application Reference

Lee K et al. JCI Insight 2023; 8 (12) T cell metabolic reprogramming in acute kidney injury and protection by glutamine blockade.
Brigitte Potier et al. Int J Mol Sci 2022; 23 (2) DM1 Transgenic Mice Exhibit Abnormal Neurotransmitter Homeostasis and Synaptic Plasticity in Association with RNA Foci and Mis-Splicing in the Hippocampus.
Application : ICC/IF
Reactivity : Mouse
Potier B et al. Int. J. Mol. Sci. 2022; 23 (2) : 592 DM1 Transgenic Mice Exhibit Abnormal Neurotransmitter Homeostasis and Synaptic Plasticity in Association with RNA Foci and Mis-Splicing in the Hippocampus.
Application : ICC/IF
Reactivity : Mouse
Bruntz RC et al. Proteomics 2019; : e1800486 Inhibition of anaplerotic glutaminolysis underlies selenite toxicity in human lung cancer.
Application : WB
Reactivity : Human
Johnson MO et al. Cell 2018; 175 (7) : 1780-1795.e19 Distinct Regulation of Th17 and Th1 Cell Differentiation by Glutaminase-Dependent Metabolism.
Application : WB
Reactivity : Mouse
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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399