FACS analysis of U-2 OS cells using GTX81012 GLS antibody, C-term.
Green : GLS
Blue : Isotype control
Fixation : 2% paraformaldehyde (PFA) (10 min)
Permeabilization : 90% methanol (10 min)
Dilution : 1:25

ICC/IF analysis of HeLa cells using GTX81012 GLS antibody, C-term.
Green : GLS
Red : Actin filament
Blue : cell nucleus
Dilution : 1:25

IHC-P analysis of human kidney tissue using GTX81012 GLS antibody, C-term.
Antigen retrieval : heat-induced antigen retrieval with a citrate buffer (pH6)
Dilution : 1:25

WB analysis of various samples using GTX81012 GLS antibody, C-term.
Lane 1: human brain lysate
Lane 2: human kidney lysate
Lane 3: mouse brain lysate
Loading : 20 μg per lane
Dilution : 1:2000

ICC/IF analysis of HeLa cells using GTX81012 GLS antibody, C-term.
Green : GLS
Red : Actin filament
Blue : cell nucleus
Dilution : 1:25

WB analysis of various samples using GTX81012 GLS antibody, C-term.
Lane 1: human brain lysate
Lane 2: mouse brain lysate
Lane 3: 293T/17 whole cell lysate
Loading : 20 μg per lane
Dilution : 1:2000

IHC-P analysis of human brain tissue using GTX81012 GLS antibody, C-term.
Antigen retrieval : heat-induced antigen retrieval with a citrate buffer (pH6)
Dilution : 1:25

The data was published in the 2022 in Int J Mol Sci. PMID: 35054778