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Glutaminase antibody [C1C3]

Anti-Glutaminase antibody [C1C3] used in Western Blot (WB). GTX114982

Cat. No. GTX114982

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB

Reactivity

Mouse
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

73 kDa. ( Note )

Positive Control

Raw264.7

Predict Reactivity

Human, Rat, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.71 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Glutaminase. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10623577

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glutaminase , AAD20 , CASGID , EIEE71 , GAC , GAM , GLS1 , KGA

Cellular Localization

Mitochondrion

Background

Sahai (1983) [PubMed 6825316] demonstrated phosphate-activated glutaminase (EC 3.5.1.2) in human platelets. It is the major enzyme yielding glutamate from glutamine. Significance of the enzyme derives from its possible implication in behavior disturbances in which glutamate acts as a neurotransmitter (Prusiner, 1981). High heritability of platelet glutaminase was indicated by studies of Sahai and Vogel (1983) [PubMed 6682827] who found an intraclass correlation coefficient of 0.96 for monozygotic twins and 0.53 for dizygotic twins.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-Glutaminase antibody [C1C3] used in Western Blot (WB). GTX114982

GTX114982 WB Image

Glutaminase antibody [C1C3] detects Glutaminase protein by Western blot analysis.
A. 20 μg Raw264 whole cell lysate/extract
Glutaminase antibody [C1C3] (GTX114982) dilution: 1:1000

REFERENCE

There are currently no references for Glutaminase antibody [C1C3] (GTX114982). Be the first to share your publications with this product.

REVIEW

Glutaminase antibody [C1C3] Cat. No. GTX114982

Rating ( Average 4 based on 1 users reviews)
Application
Western Blot(WB) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 08-Jul-2013
Score :
Application Tested : WB
Sample Species : Ms
Sample : RAW264 cells
Amount used : 20μg
Blocking : TBS-TWEEN 0.05%+MILK 5%, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169