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United States (US)

Glutaminase antibody [C1C3]

GTX114982_40240_CT_WB_M_18121410_102.jpg

Cat No. GTX114982

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

73 kDa. ( Note )

Positive Control

RAW264

Predict Reactivity

Human, Rat, Chicken(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.71 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Glutaminase. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Glutaminase,Aad20,Gac,Gam,Gls1,Kga,Gls

Cellular Localization

Mitochondrion

Background

Sahai (1983) [PubMed 6825316] demonstrated phosphate-activated glutaminase (EC 3.5.1.2) in human platelets. It is the major enzyme yielding glutamate from glutamine. Significance of the enzyme derives from its possible implication in behavior disturbances in which glutamate acts as a neurotransmitter (Prusiner, 1981). High heritability of platelet glutaminase was indicated by studies of Sahai and Vogel (1983) [PubMed 6682827] who found an intraclass correlation coefficient of 0.96 for monozygotic twins and 0.53 for dizygotic twins.[supplied by OMIM]

Database

Research Area

DATA IMAGES
GTX114982_40240_CT_WB_M_18121410_102.jpg

GTX114982 WB Image

Glutaminase antibody [C1C3] detects Glutaminase protein by Western blot analysis.
A. 20 µg Raw264 whole cell lysate/extract
Glutaminase antibody [C1C3] (GTX114982) dilution: 1:1000

REVIEW

Glutaminase antibody [C1C3] Cat No. GTX114982

Rating ( Average 4 based on 1 users reviews)
Application
Western Blot(WB) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 08-Jul-2013
Score :
Application Tested : WB
Sample Species : Ms
Sample : RAW264 cells
Amount used : 20μg
Blocking : TBS-TWEEN 0.05%+MILK 5%, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, overnightHr
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169