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Glutamine synthetase antibody [1F4]

Overexpression
Anti-Glutamine synthetase antibody [1F4] used in IHC (Paraffin sections) (IHC-P). GTX84426
Anti-Glutamine synthetase antibody [1F4] used in Flow cytometry (FACS). GTX84426
Anti-Glutamine synthetase antibody [1F4] used in Western Blot (WB). GTX84426
Anti-Glutamine synthetase antibody [1F4] used in IHC (Paraffin sections) (IHC-P). GTX84426
Anti-Glutamine synthetase antibody [1F4] used in Flow cytometry (FACS). GTX84426
Anti-Glutamine synthetase antibody [1F4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84426
Anti-Glutamine synthetase antibody [1F4] used in Western Blot (WB). GTX84426
Anti-Glutamine synthetase antibody [1F4] used in IHC (Paraffin sections) (IHC-P). GTX84426
Anti-Glutamine synthetase antibody [1F4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84426

Cat. No. GTX84426

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 1F4

Isotype

 IgG2a

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human
Package
100 μl ($519)
See all Glutamine synthetase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ICC/IF 1:50-1:100
IHC-P 1:50
FACS 1:100
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human GLUL (NP_002056) produced in HEK293T cell.

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glutamate-ammonia ligase , GLNS , GS , PIG43 , PIG59

Cellular Localization

Cytoplasm, cytosol,Microsome,Mitochondrion,Cell membrane

Background

This enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner (By similarity). Essential for proliferation of fetal skin fibroblasts.

Database

Research Area

DATA IMAGES

Anti-Glutamine synthetase antibody [1F4] used in IHC (Paraffin sections) (IHC-P). GTX84426

GTX84426 IHC-P Image

IHC-P analysis of human endometrium tissue using GTX84426 Glutamine synthetase antibody [1F4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-Glutamine synthetase antibody [1F4] used in Flow cytometry (FACS). GTX84426

GTX84426 FACS Image

FACS analysis of Jurkat cells using GTX84426 Glutamine synthetase antibody [1F4].
Red : Primary antibody
Blue : Negative control antibody

Anti-Glutamine synthetase antibody [1F4] used in Western Blot (WB). GTX84426

GTX84426 WB Image

Overexpression

WB analysis of HEK293T cells transfected with Glutamine synthetase plasmid (Right) or empty vector (Left) for 48 hrs using GTX84426 Glutamine synthetase antibody [1F4].
Loading : 5 ug per lane

Anti-Glutamine synthetase antibody [1F4] used in IHC (Paraffin sections) (IHC-P). GTX84426

GTX84426 IHC-P Image

IHC-P analysis of human breast tissue using GTX84426 Glutamine synthetase antibody [1F4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-Glutamine synthetase antibody [1F4] used in Flow cytometry (FACS). GTX84426

GTX84426 FACS Image

FACS analysis of HeLa cells using GTX84426 Glutamine synthetase antibody [1F4].
Red : Primary antibody
Blue : Negative control antibody

Anti-Glutamine synthetase antibody [1F4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84426

GTX84426 ICC/IF Image

ICC/IF analysis of HepG2 cells using GTX84426 Glutamine synthetase antibody [1F4].

Anti-Glutamine synthetase antibody [1F4] used in Western Blot (WB). GTX84426

GTX84426 WB Image

WB analysis of various cell lines using GTX84426 Glutamine synthetase antibody [1F4].
Loading : 35 ug per lane

Anti-Glutamine synthetase antibody [1F4] used in IHC (Paraffin sections) (IHC-P). GTX84426

GTX84426 IHC-P Image

IHC-P analysis of human ovary tissue using GTX84426 Glutamine synthetase antibody [1F4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-Glutamine synthetase antibody [1F4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84426

GTX84426 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with Glutamine synthetase plasmid using GTX84426 Glutamine synthetase antibody [1F4].

REFERENCE

Application Reference

Guenther SR et al. Graefes Arch Clin Exp Ophthalmol 2021; Cell composition at the vitreomacular interface in traumatic macular holes.
Application : IHC-P
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519